Detailed information of TA system TA194509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 30009..30637
• Replicon: plasmid unnamed5
• Accession: NZ_CP070614
• Organism: Rhodococcus sp. R79

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JWS13_RS00140
• Protein ID: WP_206003893.1
• Coordinates: 30227..30637 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JWS13_RS00135
• Protein ID: WP_206003892.1
• Coordinates: 30009..30230 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JWS13_RS00110	25142..25225	-	84	Protein_21	zinc-ribbon domain-containing protein	-
JWS13_RS00115	25307..25738	+	432	WP_012687137.1	hypothetical protein	-
JWS13_RS00120	25741..26793	+	1053	WP_012687136.1	IS3 family transposase	-
JWS13_RS00125	26753..27592	-	840	WP_206003891.1	zinc-ribbon domain-containing protein	-
JWS13_RS00130	29009..29638	+	630	WP_206004214.1	hypothetical protein	-
JWS13_RS00135	30009..30230	+	222	WP_206003892.1	ribbon-helix-helix protein, CopG family	Antitoxin
JWS13_RS00140	30227..30637	+	411	WP_206003893.1	PIN domain-containing protein	Toxin
JWS13_RS00145	31181..32659	-	1479	WP_206003894.1	plasmid pRiA4b ORF-3 family protein	-
JWS13_RS00150	33132..34379	+	1248	WP_206003895.1	zinc-ribbon domain-containing protein	-
JWS13_RS00155	34773..35234	+	462	WP_206003896.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..468864	468864

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-124)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-40)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15072.28 Da
• Isoelectric Point: 6.4820

>T194509 WP_206003893.1 NZ_CP070614:30227-30637 [Rhodococcus sp. R79]
MSDPTWLIDKSAMVRLGASPDAEQWANRIQRGLVHICTVTLLEVGYSARTAHDLTRVRRTPPLSAMPLEYSTPAIEDRAL
DVQRQLAERGQHRAPSIPDLLIAATAEISNRVVLHLDKDFDLIAAITGQPVERLVT

Nucleotide

• Length: 411 bp

>T194509 NZ_CP093536:c52581-52276 [Ralstonia pseudosolanacearum]
ATGACGATGGCCCAACGCACCATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATCGCCGC
CTACCTCGACGCCTGCCTGGAAGAAGCCGGAGATGACCCCGCCTTCATCACGCATGCGCTGGGCGTGGTCGCGCGCTCGC
GCGGCATGACGCAGCTCGCCCGCGAGACCGGGATGACCCGTGAAGGGCTGTACAAGGCGCTTTCCGAGGGCGGCAATCCG
AGCTTTGCGACCGTGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGACCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8349.36 Da
• Isoelectric Point: 4.5238

>AT194509 WP_206003892.1 NZ_CP070614:30009-30230 [Rhodococcus sp. R79]
MSDVLIRNFPAEDLALLDEHAARLGISRTEYLRRQLQQEARRTITTVSAADLVRFSQDFPDLADDEVMRGAWS

Nucleotide

• Length: 222 bp

>AT194509 NZ_CP093536:c52805-52578 [Ralstonia pseudosolanacearum]
GTGCGCATCGACCGCCTGCAGATGGGCCACCCGGGCGACATGAAAGCGGTCCGTGACGGCATCCGTGAGCTGCGGATCGA
TCATGGGCCGGGCGACCGCTTGTACTTCGTGCGGCATGGCGTGGTCCTGATCGTACTGCTCTGCGGCGGCGACAAGTCGA
CGCAGGAAACCGATATCCGTCGCGCAATTGACCTGTCCCGCCGTCTCGACGTGGACGACATGGAATGA