Detailed information of TA system TA194496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 4507752..4508342
• Replicon: chromosome
• Accession: NZ_CP070602
• Organism: Klebsiella pneumoniae strain KPA9853

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: JW307_RS22880
• Protein ID: WP_023341911.1
• Coordinates: 4508010..4508342 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: JW307_RS22875
• Protein ID: WP_000288812.1
• Coordinates: 4507752..4508009 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW307_RS22855	4504087..4505835	+	1749	WP_032418698.1	hypothetical protein	-
JW307_RS22860	4505889..4506470	+	582	WP_099184326.1	hypothetical protein	-
JW307_RS22865	4506753..4507214	+	462	WP_004213450.1	hypothetical protein	-
JW307_RS22870	4507211..4507417	+	207	WP_020805021.1	helix-turn-helix domain-containing protein	-
JW307_RS22875	4507752..4508009	+	258	WP_000288812.1	antitoxin	Antitoxin
JW307_RS22880	4508010..4508342	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JW307_RS22890	4508664..4510100	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
JW307_RS22900	4510466..4511920	-	1455	WP_004148975.1	AMP nucleosidase	-
JW307_RS22905	4512050..4512295	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T194496 WP_023341911.1 NZ_CP070602:4508010-4508342 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T194496 NZ_CP093528:763853-764116 [Shinella zoogloeoides]
ATGAGCTTTGAATACGATCCCGAAAAGAGCAGGTCGAACAAGGCCAAGCACGGCATTGATTTCGATGAAGTGCAGGCGCT
CTGGCAAGATGAGCGCCTCATCGAGGCGCCGGCACGCACGGACGATGAGCCGCGATACCTTGCCGTCGGCATGATCGATG
GCCGGCACTGGGCAGCCGTTTGCGTTCGCCGGGGCGACAGCGTGCGCATCATTTCCGTCAGGCGGGCACGCGAACAGGAG
ATAGAATATTATGAAAGCGACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT194496 WP_000288812.1 NZ_CP070602:4507752-4508009 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT194496 NZ_CP093528:764103-764318 [Shinella zoogloeoides]
ATGAAAGCGACTGAATTCGAGGCCAAGTTCGATGCCGGTGACGACGTCGCAGCCGATGTCGACTGGAGCAAAGCCCGCCG
TGCGAACCTGGAGATGCGCAGGGTCAATGTCGATTTCCCGGCATGGGTGGTTGAGGGGCTGGATCGGCAGGCGAGTCGGC
TTGGCGTAACCCGCCAGTCCCTCATCAAGATGTGGATTGCCGAAAAGCTGGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0