Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2616839..2617464 Replicon chromosome
Accession NZ_CP070602
Organism Klebsiella pneumoniae strain KPA9853

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JW307_RS13675 Protein ID WP_002882817.1
Coordinates 2616839..2617222 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JW307_RS13680 Protein ID WP_004150355.1
Coordinates 2617222..2617464 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW307_RS13660 2614205..2615107 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JW307_RS13665 2615104..2615739 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JW307_RS13670 2615736..2616665 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JW307_RS13675 2616839..2617222 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JW307_RS13680 2617222..2617464 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JW307_RS13685 2617669..2618586 + 918 WP_032418237.1 alpha/beta hydrolase -
JW307_RS13690 2618600..2619541 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JW307_RS13695 2619586..2620023 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JW307_RS13700 2620020..2620880 - 861 WP_004146232.1 virulence factor BrkB family protein -
JW307_RS13705 2620874..2621473 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T194493 WP_002882817.1 NZ_CP070602:c2617222-2616839 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T194493 NZ_CP093527:1823910-1824098 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATATTCGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT194493 WP_004150355.1 NZ_CP070602:c2617464-2617222 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT194493 NZ_CP093527:1823621-1823881 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References