Detailed information of TA system TA194486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 73410..74053
• Replicon: plasmid pA9853_KPC
• Accession: NZ_CP070599
• Organism: Klebsiella pneumoniae strain KPA9853

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: JW307_RS00420
• Protein ID: WP_001044770.1
• Coordinates: 73637..74053 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: JW307_RS00415
• Protein ID: WP_001261282.1
• Coordinates: 73410..73640 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW307_RS00380	68982..69848	-	867	WP_004118283.1	replication initiation protein	-
JW307_RS00385	70383..70487	+	105	WP_032409716.1	hypothetical protein	-
JW307_RS00390	70616..70873	+	258	WP_000764642.1	hypothetical protein	-
JW307_RS00395	70931..71707	-	777	WP_000015958.1	site-specific integrase	-
JW307_RS00400	71704..72447	-	744	WP_000129823.1	hypothetical protein	-
JW307_RS00405	72498..72848	-	351	WP_000493378.1	hypothetical protein	-
JW307_RS00410	72992..73453	-	462	WP_072202616.1	hypothetical protein	-
JW307_RS00415	73410..73640	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JW307_RS00420	73637..74053	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
JW307_RS00425	74127..74837	+	711	WP_004152334.1	ATP-binding protein	-
JW307_RS00430	74871..75568	+	698	Protein_85	IS1 family transposase	-
JW307_RS00435	75584..75694	+	111	Protein_86	mercuric transport protein periplasmic component	-
JW307_RS00440	75730..76152	+	423	WP_001340589.1	organomercurial transporter MerC	-
JW307_RS00445	76204..77898	+	1695	WP_000105636.1	mercury(II) reductase	-
JW307_RS00450	77916..78278	+	363	WP_001277456.1	mercury resistance co-regulator MerD	-
JW307_RS00455	78275..78511	+	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	armA / msr(E) / mph(E) / blaTEM-1A / blaOXA-9 / blaKPC-2	-	1..122733	122733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T194486 WP_001044770.1 NZ_CP070599:73637-74053 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T194486 NZ_CP093503:c6379-5963 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT194486 WP_001261282.1 NZ_CP070599:73410-73640 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT194486 NZ_CP093503:c6606-6376 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3