Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 97840..98576 Replicon plasmid pA4411_IncFII
Accession NZ_CP070595
Organism Klebsiella pneumoniae strain KPA4411

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag JW306_RS00995 Protein ID WP_003026803.1
Coordinates 97840..98322 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag JW306_RS01000 Protein ID WP_003026799.1
Coordinates 98310..98576 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW306_RS00980 93477..94439 + 963 WP_004152113.1 M48 family metalloprotease -
JW306_RS00985 94527..95781 + 1255 Protein_92 IS3 family transposase -
JW306_RS00990 96282..97628 - 1347 WP_020314316.1 ISNCY family transposase -
JW306_RS00995 97840..98322 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
JW306_RS01000 98310..98576 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
JW306_RS01005 98752..99006 - 255 WP_004152108.1 hypothetical protein -
JW306_RS01010 99082..99339 - 258 WP_004152107.1 hypothetical protein -
JW306_RS01015 99388..99591 - 204 WP_004152106.1 hemolysin expression modulator Hha -
JW306_RS01020 99625..99993 - 369 WP_004152105.1 hypothetical protein -
JW306_RS01025 100037..100531 - 495 WP_004152104.1 DNA-binding protein -
JW306_RS01030 100562..101137 - 576 WP_004152103.1 hypothetical protein -
JW306_RS01035 101125..101394 - 270 WP_004152102.1 hypothetical protein -
JW306_RS01040 101752..102102 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
JW306_RS01045 102152..102514 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid catA1 / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / qnrB4 / blaDHA-1 - 1..189174 189174


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T194466 WP_003026803.1 NZ_CP070595:c98322-97840 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T194466 NZ_CP093495:1955438-1955540 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT194466 WP_003026799.1 NZ_CP070595:c98576-98310 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT194466 NZ_CP093495:c1955547-1955402 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References