Detailed information of TA system TA194465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 42847..43490
• Replicon: plasmid pA4411_KPC
• Accession: NZ_CP070594
• Organism: Klebsiella pneumoniae strain KPA4411

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: JW306_RS00285
• Protein ID: WP_000754566.1
• Coordinates: 42847..43263 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: JW306_RS00290
• Protein ID: WP_001261276.1
• Coordinates: 43260..43490 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW306_RS00255	38024..38194	-	171	Protein_50	serine hydrolase	-
JW306_RS00260	38249..38953	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JW306_RS00265	39018..39542	+	525	Protein_52	Tn3 family transposase	-
JW306_RS00270	39705..39788	-	84	Protein_53	SOS response-associated peptidase	-
JW306_RS00275	40429..41505	-	1077	WP_000227969.1	IS110 family transposase	-
JW306_RS00280	41787..42643	-	857	Protein_55	IS3-like element ISEc15 family transposase	-
JW306_RS00285	42847..43263	-	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
JW306_RS00290	43260..43490	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JW306_RS00295	44064..44414	+	351	WP_000493378.1	hypothetical protein	-
JW306_RS00300	44465..45208	+	744	WP_000129823.1	hypothetical protein	-
JW306_RS00305	45205..45981	+	777	WP_000015958.1	site-specific integrase	-
JW306_RS00310	46039..46296	-	258	WP_000764642.1	hypothetical protein	-
JW306_RS00315	46425..46529	-	105	WP_032409716.1	hypothetical protein	-
JW306_RS00320	47059..47925	+	867	WP_004118283.1	replication initiation protein	-
JW306_RS00325	48102..48371	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / mph(A) / blaKPC-2 / aac(6')-Ib	-	1..88946	88946

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T194465 WP_000754566.1 NZ_CP070594:c43263-42847 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T194465 NZ_CP093495:874518-874928 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT194465 WP_001261276.1 NZ_CP070594:c43490-43260 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT194465 NZ_CP093495:874271-874537 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023