Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4324074..4324874 Replicon chromosome
Accession NZ_CP070593
Organism Escherichia coli strain ESC53083

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag JW305_RS22420 Protein ID WP_000342452.1
Coordinates 4324074..4324601 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag JW305_RS22425 Protein ID WP_001277107.1
Coordinates 4324608..4324874 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW305_RS22395 4319150..4319917 - 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
JW305_RS22400 4319914..4321191 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
JW305_RS22405 4321188..4322114 - 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
JW305_RS22410 4322162..4323271 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
JW305_RS22415 4323694..4324077 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
JW305_RS22420 4324074..4324601 - 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
JW305_RS22425 4324608..4324874 - 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
JW305_RS22430 4325023..4326126 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
JW305_RS22435 4326397..4327251 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
JW305_RS22440 4327496..4328554 - 1059 WP_001042013.1 permease-like cell division protein FtsX -
JW305_RS22445 4328547..4329215 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T194463 WP_000342452.1 NZ_CP070593:c4324601-4324074 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T194463 NZ_CP093495:352758-353105 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT194463 WP_001277107.1 NZ_CP070593:c4324874-4324608 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT194463 NZ_CP093495:353105-353404 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References