Detailed information of TA system TA194463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4324074..4324874
• Replicon: chromosome
• Accession: NZ_CP070593
• Organism: Escherichia coli strain ESC53083

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4T503
• Locus tag: JW305_RS22420
• Protein ID: WP_000342452.1
• Coordinates: 4324074..4324601 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4T504
• Locus tag: JW305_RS22425
• Protein ID: WP_001277107.1
• Coordinates: 4324608..4324874 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW305_RS22395	4319150..4319917	-	768	WP_000082099.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
JW305_RS22400	4319914..4321191	-	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
JW305_RS22405	4321188..4322114	-	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
JW305_RS22410	4322162..4323271	-	1110	WP_001296485.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
JW305_RS22415	4323694..4324077	+	384	WP_000778796.1	aspartate 1-decarboxylase autocleavage activator PanM	-
JW305_RS22420	4324074..4324601	-	528	WP_000342452.1	GNAT family N-acetyltransferase	Toxin
JW305_RS22425	4324608..4324874	-	267	WP_001277107.1	DUF1778 domain-containing protein	Antitoxin
JW305_RS22430	4325023..4326126	-	1104	WP_001021994.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
JW305_RS22435	4326397..4327251	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
JW305_RS22440	4327496..4328554	-	1059	WP_001042013.1	permease-like cell division protein FtsX	-
JW305_RS22445	4328547..4329215	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.57 Da
• Isoelectric Point: 6.6348

>T194463 WP_000342452.1 NZ_CP070593:c4324601-4324074 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Nucleotide

• Length: 528 bp

>T194463 NZ_CP093495:352758-353105 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9929.38 Da
• Isoelectric Point: 10.0863

>AT194463 WP_001277107.1 NZ_CP070593:c4324874-4324608 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT194463 NZ_CP093495:353105-353404 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A061K5K9

Antitoxin

• AlphaFold DB: A0A061YQ57