Detailed information of TA system TA194460

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3815667..3816501
• Replicon: chromosome
• Accession: NZ_CP070593
• Organism: Escherichia coli strain ESC53083

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: JW305_RS19895
• Protein ID: WP_000854690.1
• Coordinates: 3816124..3816501 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: JW305_RS19890
• Protein ID: WP_001305076.1
• Coordinates: 3815667..3816035 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW305_RS19850	3810749..3811876	+	1128	Protein_3622	hypothetical protein	-
JW305_RS19855	3811952..3812407	+	456	WP_000581502.1	hypothetical protein	-
JW305_RS19860	3812486..3812719	+	234	WP_000902034.1	DUF905 family protein	-
JW305_RS19865	3812820..3813638	+	819	WP_001234620.1	DUF945 domain-containing protein	-
JW305_RS19870	3813693..3814178	+	486	WP_000849565.1	antirestriction protein	-
JW305_RS19875	3814194..3814670	+	477	WP_001186726.1	RadC family protein	-
JW305_RS19880	3814733..3814954	+	222	WP_000692329.1	DUF987 domain-containing protein	-
JW305_RS19885	3814973..3815617	+	645	WP_000094916.1	hypothetical protein	-
JW305_RS19890	3815667..3816035	+	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JW305_RS19895	3816124..3816501	+	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
JW305_RS19900	3816498..3816986	+	489	WP_000761699.1	hypothetical protein	-
JW305_RS19905	3817003..3817200	+	198	WP_000839293.1	DUF957 domain-containing protein	-
JW305_RS19910	3817285..3818130	+	846	WP_001529401.1	DUF4942 domain-containing protein	-
JW305_RS19915	3818199..3818594	+	396	WP_000208384.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
JW305_RS19920	3818587..3819520	+	934	Protein_3636	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JW305_RS19925	3819952..3820107	+	156	WP_000729638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3819952..3820107	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T194460 WP_000854690.1 NZ_CP070593:3816124-3816501 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T194460 NZ_CP093489:c5043464-5043081 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT194460 WP_001305076.1 NZ_CP070593:3815667-3816035 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT194460 NZ_CP093489:c5043706-5043464 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8