Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2754024..2754855 Replicon chromosome
Accession NZ_CP070593
Organism Escherichia coli strain ESC53083

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag JW305_RS15020 Protein ID WP_000854815.1
Coordinates 2754481..2754855 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag JW305_RS15015 Protein ID WP_001280918.1
Coordinates 2754024..2754392 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW305_RS14970 2749113..2749859 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
JW305_RS14975 2749942..2750292 + 351 Protein_2671 hypothetical protein -
JW305_RS14980 2750308..2750718 + 411 WP_000846703.1 hypothetical protein -
JW305_RS14985 2750939..2751757 + 819 WP_001542275.1 DUF945 domain-containing protein -
JW305_RS14990 2751757..2752002 + 246 WP_001164966.1 hypothetical protein -
JW305_RS14995 2752096..2752569 + 474 WP_001542276.1 antirestriction protein -
JW305_RS15000 2752585..2753061 + 477 WP_001186200.1 RadC family protein -
JW305_RS15005 2753124..2753345 + 222 WP_000692345.1 DUF987 domain-containing protein -
JW305_RS15010 2753364..2754008 + 645 WP_000086752.1 hypothetical protein -
JW305_RS15015 2754024..2754392 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
JW305_RS15020 2754481..2754855 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
JW305_RS15025 2754852..2755046 + 195 WP_000988600.1 hypothetical protein -
JW305_RS15030 2755059..2755172 + 114 WP_001161660.1 DUF957 domain-containing protein -
JW305_RS15035 2755461..2755607 - 147 Protein_2683 transposase domain-containing protein -
JW305_RS15040 2755661..2755843 + 183 WP_001296208.1 hypothetical protein -
JW305_RS15045 2755944..2756273 - 330 WP_000450409.1 DUF496 family protein -
JW305_RS15050 2756445..2757503 - 1059 WP_001200889.1 FUSC family protein -
JW305_RS15055 2757701..2758174 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
JW305_RS15060 2758293..2759459 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T194457 WP_000854815.1 NZ_CP070593:2754481-2754855 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T194457 NZ_CP093489:c4521398-4521114 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGCCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCAGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT194457 WP_001280918.1 NZ_CP070593:2754024-2754392 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT194457 NZ_CP093489:c4521630-4521388 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References