Detailed information of TA system TA194441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 837775..839464
• Replicon: chromosome
• Accession: NZ_CP070593
• Organism: Escherichia coli strain ESC53083

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: JW305_RS05340
• Protein ID: WP_000365807.1
• Coordinates: 838544..839464 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: JW305_RS05335
• Protein ID: WP_001295795.1
• Coordinates: 837775..838557 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW305_RS05330	834393..837491	-	3099	WP_000673070.1	type I restriction endonuclease subunit R	-
JW305_RS05335	837775..838557	+	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
JW305_RS05340	838544..839464	+	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JW305_RS05345	839629..840948	-	1320	Protein_778	site-specific integrase	-
JW305_RS05350	841175..841333	-	159	Protein_779	integrase	-
JW305_RS05355	841382..841567	+	186	WP_000146240.1	hypothetical protein	-
JW305_RS05360	841782..842396	+	615	WP_001019923.1	YagU family protein	-
JW305_RS05365	842470..842633	+	164	Protein_782	tail fiber assembly protein	-
JW305_RS05370	842645..842974	-	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
JW305_RS05375	843281..843991	-	711	WP_001295798.1	fimbrial chaperone EcpE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T194441 WP_000365807.1 NZ_CP070593:838544-839464 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

>T194441 NZ_CP093486:1926117-1926219 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT194441 WP_001295795.1 NZ_CP070593:837775-838557 [Escherichia coli]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT194441 NZ_CP093486:c1926226-1926081 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A061KTE0