Detailed information of TA system TA194433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 58018..58661
• Replicon: plasmid p53083_KPC
• Accession: NZ_CP070587
• Organism: Escherichia coli strain ESC53083

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: JW305_RS00355
• Protein ID: WP_000754566.1
• Coordinates: 58018..58434 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: JW305_RS00360
• Protein ID: WP_001261276.1
• Coordinates: 58431..58661 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW305_RS00325	53195..53365	-	171	Protein_64	serine hydrolase	-
JW305_RS00330	53420..54124	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JW305_RS00335	54189..54713	+	525	Protein_66	Tn3 family transposase	-
JW305_RS00340	54876..54959	-	84	Protein_67	SOS response-associated peptidase	-
JW305_RS00345	55600..56676	-	1077	WP_000227969.1	IS110 family transposase	-
JW305_RS00350	56958..57814	-	857	Protein_69	IS3-like element ISEc15 family transposase	-
JW305_RS00355	58018..58434	-	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
JW305_RS00360	58431..58661	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JW305_RS00365	59235..59585	+	351	WP_000493378.1	hypothetical protein	-
JW305_RS00370	59636..60379	+	744	WP_000129823.1	hypothetical protein	-
JW305_RS00375	60376..61152	+	777	WP_000015958.1	site-specific integrase	-
JW305_RS00380	61210..61467	-	258	WP_000764642.1	hypothetical protein	-
JW305_RS00385	61596..61700	-	105	WP_032409716.1	hypothetical protein	-
JW305_RS00390	62230..63096	+	867	WP_004118283.1	replication initiation protein	-
JW305_RS00395	63273..63542	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / blaKPC-3 / aac(6')-Ib / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1	-	1..88947	88947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T194433 WP_000754566.1 NZ_CP070587:c58434-58018 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T194433 NZ_CP093481:4965963-4966340 [Klebsiella pneumoniae]
ATGAAAACATTACCCGACACACACGAACGGGAGGCGTCACGCTGCTCGTCTCCTGTGACAATCTGGCAGACACTCCTGGC
CCGGCTGCTGGGCCAGCATTACAGCCTCACGCTGAATGACACGCCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAGTACGCGCTGGTACGTACCGACCAGCCGGGATTC
GGCACCTGTGCCCATTCGCAGTTAATAAACAGCATCGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGTTGACCCGCAG
CAACTACAGAACGGTGAACGACATTATCCGGGGTAAAGATGCGGAGGCAAAACAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT194433 WP_001261276.1 NZ_CP070587:c58661-58431 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT194433 NZ_CP093481:4965539-4965916 [Klebsiella pneumoniae]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAGGGTAACCGGTTGCATTACCTTGCAGATCGCGCAGGTATAAGAGGTCAGT
TCAGCAACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGCTGAAACAACTGGAGTTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
GGGTTACGTTTATATGGCTGTTTATCCGACACCGGCAGCTCCCGCAACAACCGCATAA

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023