Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5269205..5269830 Replicon chromosome
Accession NZ_CP070586
Organism Klebsiella pneumoniae strain KP52813

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag JW304_RS27930 Protein ID WP_019705794.1
Coordinates 5269205..5269588 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JW304_RS27935 Protein ID WP_004150355.1
Coordinates 5269588..5269830 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW304_RS27915 5266571..5267473 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JW304_RS27920 5267470..5268105 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JW304_RS27925 5268102..5269031 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JW304_RS27930 5269205..5269588 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
JW304_RS27935 5269588..5269830 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JW304_RS27940 5270035..5270952 + 918 WP_040189415.1 alpha/beta hydrolase -
JW304_RS27945 5270966..5271907 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JW304_RS27950 5271952..5272389 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JW304_RS27955 5272386..5273246 - 861 WP_048975889.1 virulence factor BrkB family protein -
JW304_RS27960 5273240..5273839 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T194432 WP_019705794.1 NZ_CP070586:c5269588-5269205 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T194432 NZ_CP093481:c4809213-4808929 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGCCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCAGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT194432 WP_004150355.1 NZ_CP070586:c5269830-5269588 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT194432 NZ_CP093481:c4809445-4809203 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References