Detailed information of TA system TA194427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2702515..2704103
• Replicon: chromosome
• Accession: NZ_CP070586
• Organism: Klebsiella pneumoniae strain KP52813

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JW304_RS15595
• Protein ID: WP_040245181.1
• Coordinates: 2702515..2703837 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: JW304_RS15600
• Protein ID: WP_002904790.1
• Coordinates: 2703837..2704103 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW304_RS15580	2697571..2700072	+	2502	Protein_2595	fimbrial biogenesis outer membrane usher protein	-
JW304_RS15585	2700060..2701064	+	1005	WP_048974904.1	type 1 fimbrial protein	-
JW304_RS15590	2701206..2702498	+	1293	WP_048974906.1	glycoside hydrolase family 10 protein	-
JW304_RS15595	2702515..2703837	-	1323	WP_040245181.1	type II toxin-antitoxin system HipA family toxin	Toxin
JW304_RS15600	2703837..2704103	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JW304_RS15605	2704369..2704695	-	327	WP_004143476.1	hydroxyisourate hydrolase	-
JW304_RS15610	2704692..2705192	-	501	WP_048974909.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
JW304_RS15615	2705170..2706549	-	1380	WP_004184021.1	purine permease	-
JW304_RS15620	2706895..2708049	+	1155	WP_048265552.1	FAD-dependent urate hydroxylase HpxO	-
JW304_RS15625	2708030..2708953	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49141.44 Da
• Isoelectric Point: 7.2487

>T194427 WP_040245181.1 NZ_CP070586:c2703837-2702515 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVWRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T194427 NZ_CP093481:814748-815158 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT194427 WP_002904790.1 NZ_CP070586:c2704103-2703837 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT194427 NZ_CP093481:814501-814767 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8