Detailed information of TA system TA194417

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 699416..700170
• Replicon: chromosome
• Accession: NZ_CP070586
• Organism: Klebsiella pneumoniae strain KP52813

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A6L5EGB6
• Locus tag: JW304_RS05805
• Protein ID: WP_032669201.1
• Coordinates: 699793..700170 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JW304_RS05800
• Protein ID: WP_074143921.1
• Coordinates: 699416..699742 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW304_RS05755	694644..695357	+	714	WP_032669194.1	WYL domain-containing protein	-
JW304_RS05760	695354..695989	+	636	WP_020837117.1	hypothetical protein	-
JW304_RS05765	696028..696483	+	456	WP_000754598.1	hypothetical protein	-
JW304_RS05770	696480..696929	+	450	WP_032669197.1	hypothetical protein	-
JW304_RS05775	697001..697232	+	232	Protein_676	DUF905 domain-containing protein	-
JW304_RS05780	697331..698152	+	822	WP_032669198.1	DUF945 domain-containing protein	-
JW304_RS05785	698183..698626	+	444	WP_032669199.1	antirestriction protein	-
JW304_RS05790	698638..699117	+	480	WP_032669200.1	DNA repair protein RadC	-
JW304_RS05795	699138..699359	+	222	WP_023567997.1	DUF987 domain-containing protein	-
JW304_RS05800	699416..699742	+	327	WP_074143921.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JW304_RS05805	699793..700170	+	378	WP_032669201.1	TA system toxin CbtA family protein	Toxin
JW304_RS05810	700167..700658	+	492	WP_032672023.1	hypothetical protein	-
JW304_RS05815	700688..700891	+	204	WP_032669203.1	DUF957 domain-containing protein	-
JW304_RS05820	700955..701803	+	849	WP_032669204.1	DUF4942 domain-containing protein	-
JW304_RS05830	702106..702612	+	507	WP_002917636.1	G/U mismatch-specific DNA glycosylase	-
JW304_RS05835	702712..704553	-	1842	WP_004174339.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	685057..700170	15113

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14157.93 Da
• Isoelectric Point: 6.2236

>T194417 WP_032669201.1 NZ_CP070586:699793-700170 [Klebsiella pneumoniae]
MQTQPLSSTQEATPRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLSSIDILRARKATGLMTRNDYRTVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T194417 NZ_CP093476:c4987571-4987287 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11953.71 Da
• Isoelectric Point: 6.8397

>AT194417 WP_074143921.1 NZ_CP070586:699416-699742 [Klebsiella pneumoniae]
ITEPWWGLKRSITPCFGARLVQAGNRLHYLADRANITGQFSDADLRYLDQAFPLLLKQLELMLTSGELNPCHQHCVTLYA
KGLTCEADSLGSHGYVYLAIYPTPAATA

Nucleotide

• Length: 327 bp

>AT194417 NZ_CP093476:c4987803-4987561 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A6L5EGB6