Detailed information of TA system TA194415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 365735..366321
• Replicon: chromosome
• Accession: NZ_CP070586
• Organism: Klebsiella pneumoniae strain KP52813

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A9J6Y968
• Locus tag: JW304_RS04050
• Protein ID: WP_025367836.1
• Coordinates: 365953..366321 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: JW304_RS04045
• Protein ID: WP_004174006.1
• Coordinates: 365735..365956 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW304_RS04025	361892..362818	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
JW304_RS04030	362815..364092	+	1278	WP_004188292.1	branched chain amino acid ABC transporter permease LivM	-
JW304_RS04035	364089..364856	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
JW304_RS04040	364858..365571	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
JW304_RS04045	365735..365956	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JW304_RS04050	365953..366321	+	369	WP_025367836.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
JW304_RS04055	366594..367910	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
JW304_RS04060	368017..368904	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
JW304_RS04065	368901..369746	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
JW304_RS04070	369748..370818	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	355379..371555	16176

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13449.86 Da
• Isoelectric Point: 7.2790

>T194415 WP_025367836.1 NZ_CP070586:365953-366321 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIECEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLLG

Nucleotide

• Length: 369 bp

>T194415 NZ_CP093476:4234913-4235131 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT194415 WP_004174006.1 NZ_CP070586:365735-365956 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT194415 NZ_CP093476:4234512-4234886 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A5E5YJY7