Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 54603..55339 Replicon plasmid p52813_IncFIB
Accession NZ_CP070582
Organism Klebsiella pneumoniae strain KP52813

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag JW304_RS01405 Protein ID WP_003026803.1
Coordinates 54857..55339 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag JW304_RS01400 Protein ID WP_003026799.1
Coordinates 54603..54869 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW304_RS01355 50665..51027 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
JW304_RS01360 51077..51427 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
JW304_RS01365 51785..52054 + 270 WP_004152102.1 hypothetical protein -
JW304_RS01370 52042..52617 + 576 WP_004152103.1 hypothetical protein -
JW304_RS01375 52648..53142 + 495 WP_004152104.1 DNA-binding protein -
JW304_RS01380 53186..53554 + 369 WP_004152105.1 hypothetical protein -
JW304_RS01385 53588..53791 + 204 WP_004152106.1 hemolysin expression modulator Hha -
JW304_RS01390 53840..54097 + 258 WP_004152107.1 hypothetical protein -
JW304_RS01395 54173..54427 + 255 WP_004152108.1 hypothetical protein -
JW304_RS01400 54603..54869 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
JW304_RS01405 54857..55339 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
JW304_RS01410 55498..56752 - 1255 Protein_57 IS3 family transposase -
JW304_RS01415 56840..57802 - 963 WP_023313934.1 M48 family metalloprotease -
JW304_RS01420 57789..58538 - 750 WP_205461628.1 phosphate-starvation-inducible PsiE family protein -
JW304_RS01425 58776..58973 - 198 WP_004152115.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..208206 208206


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T194413 WP_003026803.1 NZ_CP070582:54857-55339 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T194413 NZ_CP093476:2051223-2051325 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT194413 WP_003026799.1 NZ_CP070582:54603-54869 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT194413 NZ_CP093476:c2051331-2051187 [Klebsiella pneumoniae]
ACATATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References