Detailed information of TA system TA194412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 86531..87056
• Replicon: plasmid p52813_Incfii
• Accession: NZ_CP070581
• Organism: Klebsiella pneumoniae strain KP52813

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: JW304_RS01080
• Protein ID: WP_004197633.1
• Coordinates: 86751..87056 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: JW304_RS01075
• Protein ID: WP_004197642.1
• Coordinates: 86531..86749 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW304_RS01030	81637..82632	+	996	WP_019725651.1	hypothetical protein	-
JW304_RS01035	82705..83028	+	324	WP_019725650.1	hypothetical protein	-
JW304_RS01040	83462..83656	+	195	WP_019725033.1	hypothetical protein	-
JW304_RS01045	83700..83930	-	231	WP_019725034.1	hypothetical protein	-
JW304_RS01050	83944..84147	-	204	WP_019725040.1	hemolysin expression modulator Hha	-
JW304_RS01055	84181..84549	-	369	WP_015065502.1	hypothetical protein	-
JW304_RS01060	84593..85087	-	495	WP_009310053.1	hypothetical protein	-
JW304_RS01065	85118..85690	-	573	WP_015065504.1	hypothetical protein	-
JW304_RS01070	85687..85935	-	249	WP_019725036.1	hypothetical protein	-
JW304_RS01075	86531..86749	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
JW304_RS01080	86751..87056	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
JW304_RS01085	87225..87626	+	402	WP_072199347.1	hypothetical protein	-
JW304_RS01090	87653..87976	+	324	WP_004197641.1	hypothetical protein	-
JW304_RS01095	87973..88989	+	1017	WP_004197639.1	hypothetical protein	-
JW304_RS01100	89187..89981	+	795	WP_004197635.1	site-specific integrase	-
JW304_RS01105	90421..90600	-	180	WP_006788217.1	hypothetical protein	-
JW304_RS01110	90720..91346	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA14 / qnrB1 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..94665	94665

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T194412 WP_004197633.1 NZ_CP070581:86751-87056 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T194412 NZ_CP093476:906268-906678 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT194412 WP_004197642.1 NZ_CP070581:86531-86749 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT194412 NZ_CP093476:906021-906287 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5