Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 46187..46830 Replicon plasmid p52813_KPC
Accession NZ_CP070580
Organism Klebsiella pneumoniae strain KP52813

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag JW304_RS00250 Protein ID WP_000754566.1
Coordinates 46414..46830 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag JW304_RS00245 Protein ID WP_001261276.1
Coordinates 46187..46417 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW304_RS00210 41306..41575 + 270 WP_000339857.1 hypothetical protein -
JW304_RS00215 41752..42618 - 867 WP_004118283.1 replication initiation protein -
JW304_RS00220 43148..43252 + 105 WP_032409716.1 hypothetical protein -
JW304_RS00225 43381..43638 + 258 WP_000764642.1 hypothetical protein -
JW304_RS00230 43696..44472 - 777 WP_000015958.1 site-specific integrase -
JW304_RS00235 44469..45212 - 744 WP_000129823.1 hypothetical protein -
JW304_RS00240 45263..45613 - 351 WP_000493378.1 hypothetical protein -
JW304_RS00245 46187..46417 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JW304_RS00250 46414..46830 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
JW304_RS00255 47034..47890 + 857 Protein_50 IS3-like element ISEc15 family transposase -
JW304_RS00260 48172..49248 + 1077 WP_000227969.1 IS110 family transposase -
JW304_RS00265 49889..49972 + 84 Protein_52 SOS response-associated peptidase -
JW304_RS00270 50135..50659 - 525 Protein_53 Tn3 family transposase -
JW304_RS00275 50724..51428 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
JW304_RS00280 51483..51653 + 171 Protein_55 serine hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib / blaKPC-3 / mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr - 1..88945 88945


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T194411 WP_000754566.1 NZ_CP070580:46414-46830 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T194411 NZ_CP093476:770028-770513 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT194411 WP_001261276.1 NZ_CP070580:46187-46417 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT194411 NZ_CP093476:769738-770031 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References