Detailed information of TA system TA194391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 129213..129949
• Replicon: plasmid p52810_IncFII
• Accession: NZ_CP070577
• Organism: Klebsiella pneumoniae strain KP52810

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: JW303_RS01205
• Protein ID: WP_003026803.1
• Coordinates: 129213..129695 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: JW303_RS01210
• Protein ID: WP_003026799.1
• Coordinates: 129683..129949 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW303_RS01190	124850..125812	+	963	WP_004152113.1	M48 family metalloprotease	-
JW303_RS01195	125900..127154	+	1255	Protein_134	IS3 family transposase	-
JW303_RS01200	127655..129001	-	1347	WP_020314316.1	ISNCY family transposase	-
JW303_RS01205	129213..129695	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
JW303_RS01210	129683..129949	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
JW303_RS01215	130125..130379	-	255	WP_004152108.1	hypothetical protein	-
JW303_RS01220	130455..130712	-	258	WP_004152107.1	hypothetical protein	-
JW303_RS01225	130761..130964	-	204	WP_004152106.1	hemolysin expression modulator Hha	-
JW303_RS01230	130998..131366	-	369	WP_004152105.1	hypothetical protein	-
JW303_RS01235	131410..131904	-	495	WP_004152104.1	DNA-binding protein	-
JW303_RS01240	131935..132510	-	576	WP_004152103.1	hypothetical protein	-
JW303_RS01245	132498..132767	-	270	WP_004152102.1	hypothetical protein	-
JW303_RS01250	133125..133475	+	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
JW303_RS01255	133525..133887	+	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA1 / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1	-	1..170806	170806

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T194391 WP_003026803.1 NZ_CP070577:c129695-129213 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T194391 NZ_CP093465:c1215802-1215584 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT194391 WP_003026799.1 NZ_CP070577:c129949-129683 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT194391 NZ_CP093465:c1216203-1215829 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66