Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 56049..56692 Replicon plasmid p52810_KPC
Accession NZ_CP070576
Organism Klebsiella pneumoniae strain KP52810

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag JW303_RS00305 Protein ID WP_000754566.1
Coordinates 56276..56692 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag JW303_RS00300 Protein ID WP_001261276.1
Coordinates 56049..56279 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW303_RS00265 51168..51437 + 270 WP_000339857.1 hypothetical protein -
JW303_RS00270 51614..52480 - 867 WP_004118283.1 replication initiation protein -
JW303_RS00275 53010..53114 + 105 WP_032409716.1 hypothetical protein -
JW303_RS00280 53243..53500 + 258 WP_000764642.1 hypothetical protein -
JW303_RS00285 53558..54334 - 777 WP_000015958.1 site-specific integrase -
JW303_RS00290 54331..55074 - 744 WP_000129823.1 hypothetical protein -
JW303_RS00295 55125..55475 - 351 WP_000493378.1 hypothetical protein -
JW303_RS00300 56049..56279 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JW303_RS00305 56276..56692 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
JW303_RS00310 56896..57752 + 857 Protein_61 IS3-like element ISEc15 family transposase -
JW303_RS00315 58034..59110 + 1077 WP_000227969.1 IS110 family transposase -
JW303_RS00320 59751..59834 + 84 Protein_63 SOS response-associated peptidase -
JW303_RS00325 59997..60521 - 525 Protein_64 Tn3 family transposase -
JW303_RS00330 60586..61290 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
JW303_RS00335 61345..61515 + 171 Protein_66 serine hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib / blaKPC-2 / mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr - 1..88945 88945


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T194390 WP_000754566.1 NZ_CP070576:56276-56692 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T194390 NZ_CP093465:599461-599994 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT194390 WP_001261276.1 NZ_CP070576:56049-56279 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT194390 NZ_CP093465:599184-599450 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References