Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 28764..29407 Replicon plasmid p52808_KPC
Accession NZ_CP070575
Organism Proteus mirabilis strain PM52808

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag JW302_RS19910 Protein ID WP_000754566.1
Coordinates 28764..29180 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag JW302_RS19915 Protein ID WP_001261276.1
Coordinates 29177..29407 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW302_RS19880 23941..24111 - 171 Protein_34 serine hydrolase -
JW302_RS19885 24166..24870 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
JW302_RS19890 24935..25459 + 525 Protein_36 Tn3 family transposase -
JW302_RS19895 25622..25705 - 84 Protein_37 SOS response-associated peptidase -
JW302_RS19900 26346..27422 - 1077 WP_000227969.1 IS110 family transposase -
JW302_RS19905 27704..28560 - 857 Protein_39 IS3-like element ISEc15 family transposase -
JW302_RS19910 28764..29180 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
JW302_RS19915 29177..29407 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JW302_RS19920 29981..30331 + 351 WP_000493378.1 hypothetical protein -
JW302_RS19925 30382..31125 + 744 WP_000129823.1 hypothetical protein -
JW302_RS19930 31122..31898 + 777 WP_000015958.1 site-specific integrase -
JW302_RS19935 31956..32213 - 258 WP_000764642.1 hypothetical protein -
JW302_RS19940 32342..32446 - 105 WP_032409716.1 hypothetical protein -
JW302_RS19945 32976..33842 + 867 WP_004118283.1 replication initiation protein -
JW302_RS19950 34019..34288 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / mph(A) / blaKPC-2 / aac(6')-Ib - 1..88945 88945


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T194389 WP_000754566.1 NZ_CP070575:c29180-28764 [Proteus mirabilis]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T194389 NZ_CP093465:497617-497901 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT194389 WP_001261276.1 NZ_CP070575:c29407-29177 [Proteus mirabilis]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT194389 NZ_CP093465:497385-497627 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References