Detailed information of TA system TA194379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 46309..46952
• Replicon: plasmid p52260_KPC
• Accession: NZ_CP070571
• Organism: Proteus mirabilis strain PM52260

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: JW301_RS19955
• Protein ID: WP_000754566.1
• Coordinates: 46536..46952 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: JW301_RS19950
• Protein ID: WP_001261276.1
• Coordinates: 46309..46539 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW301_RS19915	41428..41697	+	270	WP_000339857.1	hypothetical protein	-
JW301_RS19920	41874..42740	-	867	WP_004118283.1	replication initiation protein	-
JW301_RS19925	43270..43374	+	105	WP_032409716.1	hypothetical protein	-
JW301_RS19930	43503..43760	+	258	WP_000764642.1	hypothetical protein	-
JW301_RS19935	43818..44594	-	777	WP_000015958.1	site-specific integrase	-
JW301_RS19940	44591..45334	-	744	WP_000129823.1	hypothetical protein	-
JW301_RS19945	45385..45735	-	351	WP_000493378.1	hypothetical protein	-
JW301_RS19950	46309..46539	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JW301_RS19955	46536..46952	+	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
JW301_RS19960	47156..48012	+	857	Protein_52	IS3-like element ISEc15 family transposase	-
JW301_RS19965	48294..49370	+	1077	WP_000227969.1	IS110 family transposase	-
JW301_RS19970	50011..50094	+	84	Protein_54	SOS response-associated peptidase	-
JW301_RS19975	50257..50781	-	525	Protein_55	Tn3 family transposase	-
JW301_RS19980	50846..51550	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JW301_RS19985	51605..51775	+	171	Protein_57	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib / blaKPC-2 / mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr	-	1..88945	88945

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T194379 WP_000754566.1 NZ_CP070571:46536-46952 [Proteus mirabilis]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T194379 NZ_CP093458:3897242-3897460 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT194379 WP_001261276.1 NZ_CP070571:46309-46539 [Proteus mirabilis]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT194379 NZ_CP093458:3896841-3897215 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023