Detailed information of TA system TA194375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3360670..3361691
• Replicon: chromosome
• Accession: NZ_CP070569
• Organism: Proteus mirabilis strain PM52260

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: JW301_RS15820
• Protein ID: WP_004249488.1
• Coordinates: 3361122..3361691 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: JW301_RS15815
• Protein ID: WP_004245528.1
• Coordinates: 3360670..3361122 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW301_RS15795	3356456..3356986	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
JW301_RS15800	3357255..3358268	+	1014	WP_004250228.1	thiamine ABC transporter substrate binding subunit	-
JW301_RS15805	3358268..3359848	+	1581	WP_004245526.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
JW301_RS15810	3359841..3360542	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
JW301_RS15815	3360670..3361122	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
JW301_RS15820	3361122..3361691	+	570	WP_004249488.1	PIN domain-containing protein	Toxin
JW301_RS15825	3361807..3364161	+	2355	WP_017628571.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21600.10 Da
• Isoelectric Point: 7.3470

>T194375 WP_004249488.1 NZ_CP070569:3361122-3361691 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKNPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T194375 NZ_CP093458:837735-838145 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT194375 WP_004245528.1 NZ_CP070569:3360670-3361122 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT194375 NZ_CP093458:837488-837754 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: B4F2H3