Detailed information of TA system TA194373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1916270..1917069
• Replicon: chromosome
• Accession: NZ_CP070569
• Organism: Proteus mirabilis strain PM52260

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E3RD10
• Locus tag: JW301_RS09135
• Protein ID: WP_004247966.1
• Coordinates: 1916545..1917069 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: B4EVS2
• Locus tag: JW301_RS09130
• Protein ID: WP_004247964.1
• Coordinates: 1916270..1916548 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW301_RS09105	1911620..1912702	+	1083	WP_004242680.1	peptide chain release factor 1	-
JW301_RS09110	1912702..1913550	+	849	WP_004247962.1	peptide chain release factor N(5)-glutamine methyltransferase	-
JW301_RS09115	1913528..1914343	+	816	WP_004242688.1	invasion regulator SirB1	-
JW301_RS09120	1914401..1915255	+	855	WP_004242689.1	3-deoxy-8-phosphooctulonate synthase	-
JW301_RS09125	1915263..1916192	+	930	WP_017628160.1	TIGR01212 family radical SAM protein	-
JW301_RS09130	1916270..1916548	+	279	WP_004247964.1	DUF1778 domain-containing protein	Antitoxin
JW301_RS09135	1916545..1917069	+	525	WP_004247966.1	hypothetical protein	Toxin
JW301_RS09140	1917150..1918850	-	1701	WP_017628159.1	C4-dicarboxylic acid transporter DauA	-
JW301_RS09150	1919814..1920755	+	942	WP_004242695.1	glyoxylate/hydroxypyruvate reductase GhrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-87)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19192.12 Da
• Isoelectric Point: 9.3561

>T194373 WP_004247966.1 NZ_CP070569:1916545-1917069 [Proteus mirabilis]
MTIPNWHEEAISKKHNRNGFNCGDAVLNQFLYRHARQNHENGSAKTYLAINNSNNIILGYYSLCPASIEFERTPELIKHG
LARHDIPVFKLARLATDLSVQGKGLGGQLLLAAGRRCLSVASELGGVALLIDAKNERVANWYISYGAIPLLDTPLSLLIS
FKTIYTALSMANKI

Nucleotide

• Length: 525 bp

>T194373 NZ_CP093458:341945-342313 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10252.82 Da
• Isoelectric Point: 5.1369

>AT194373 WP_004247964.1 NZ_CP070569:1916270-1916548 [Proteus mirabilis]
MAQALVENSERVSIRITTEDKSLLVRAASLENTNLTEFVLRNIVPVARQVIENNERLKLSEKDTLRVMELLDNPPKPNAK
LLAAAFALPDNA

Nucleotide

• Length: 279 bp

>AT194373 NZ_CP093458:341727-341948 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: B4EVS2