Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3632397..3633022 Replicon chromosome
Accession NZ_CP070568
Organism Klebsiella pneumoniae strain KP51248

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JW300_RS18670 Protein ID WP_002882817.1
Coordinates 3632397..3632780 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JW300_RS18675 Protein ID WP_004150355.1
Coordinates 3632780..3633022 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW300_RS18655 3629763..3630665 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JW300_RS18660 3630662..3631297 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JW300_RS18665 3631294..3632223 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JW300_RS18670 3632397..3632780 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JW300_RS18675 3632780..3633022 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JW300_RS18680 3633227..3634144 + 918 WP_032418237.1 alpha/beta hydrolase -
JW300_RS18685 3634158..3635099 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JW300_RS18690 3635144..3635581 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JW300_RS18695 3635578..3636438 - 861 WP_004146232.1 virulence factor BrkB family protein -
JW300_RS18700 3636432..3637031 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T194367 WP_002882817.1 NZ_CP070568:c3632780-3632397 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T194367 NZ_CP093451:5017429-5017806 [Klebsiella pneumoniae]
ATGAAAACATTACCCGACACACACGAACGGGAGGCGTCACGCTGCTCGTCTCCTGTGACAATCTGGCAGACACTCCTGGC
CCGGCTGCTGGGCCAGCATTACAGCCTCACGCTGAATGACACGCCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAGTACGCGCTGGTACGTACCGACCAGCCGGGATTC
GGCACCTGTGCCCATTCGCAGTTAATAAACAGCATCGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGTTGACCCGCAG
CAACTACAGAACGGTGAACGACATTATCCGGGGTAAAGATGCGGAGGCAAAACAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT194367 WP_004150355.1 NZ_CP070568:c3633022-3632780 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT194367 NZ_CP093451:5017005-5017382 [Klebsiella pneumoniae]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAGGGTAACCGGTTGCATTACCTTGCAGATCGCGCAGGTATAAGAGGTCAGT
TCAGCAACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGCTGAAACAACTGGAGTTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
GGGTTACGTTTATATGGCTGTTTATCCGACACCGGCAGCTCCCGCAACAACCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References