Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2437158..2437777 Replicon chromosome
Accession NZ_CP070568
Organism Klebsiella pneumoniae strain KP51248

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag JW300_RS12960 Protein ID WP_002892050.1
Coordinates 2437559..2437777 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag JW300_RS12955 Protein ID WP_002892066.1
Coordinates 2437158..2437532 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW300_RS12945 2432310..2433503 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JW300_RS12950 2433526..2436672 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
JW300_RS12955 2437158..2437532 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
JW300_RS12960 2437559..2437777 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
JW300_RS12965 2437936..2438502 + 567 WP_002892030.1 maltose O-acetyltransferase -
JW300_RS12970 2438474..2438614 - 141 WP_004147370.1 hypothetical protein -
JW300_RS12975 2438635..2439105 + 471 WP_002892026.1 YlaC family protein -
JW300_RS12980 2439080..2440531 - 1452 WP_032419337.1 PLP-dependent aminotransferase family protein -
JW300_RS12985 2440632..2441330 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
JW300_RS12990 2441327..2441467 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
JW300_RS12995 2441467..2441730 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T194364 WP_002892050.1 NZ_CP070568:2437559-2437777 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T194364 NZ_CP093451:4158326-4158544 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT194364 WP_002892066.1 NZ_CP070568:2437158-2437532 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT194364 NZ_CP093451:4157925-4158299 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References