Detailed information of TA system TA194353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 159734..160324
• Replicon: chromosome
• Accession: NZ_CP070568
• Organism: Klebsiella pneumoniae strain KP51248

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: JW300_RS01755
• Protein ID: WP_023341911.1
• Coordinates: 159992..160324 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: JW300_RS01750
• Protein ID: WP_000288812.1
• Coordinates: 159734..159991 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW300_RS01730	156069..157817	+	1749	WP_032418698.1	hypothetical protein	-
JW300_RS01735	157871..158452	+	582	WP_099184326.1	hypothetical protein	-
JW300_RS01740	158735..159196	+	462	WP_004213450.1	hypothetical protein	-
JW300_RS01745	159193..159399	+	207	WP_020805021.1	helix-turn-helix domain-containing protein	-
JW300_RS01750	159734..159991	+	258	WP_000288812.1	antitoxin	Antitoxin
JW300_RS01755	159992..160324	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JW300_RS01765	160646..162082	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
JW300_RS01775	162448..163902	-	1455	WP_004148975.1	AMP nucleosidase	-
JW300_RS01780	164032..164277	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T194353 WP_023341911.1 NZ_CP070568:159992-160324 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T194353 NZ_CP093445:3731983-3732336 [Salmonella enterica subsp. enterica serovar Abeokuta]
ATGCAAACCCAATACAACCACCCACACCGGGCGACCCCGTCACAGCCGTCGCCTGTCGAAATCTGGCAAAAACTGCTGAC
CCATTTACTGGCTAAACATTATGGCCTCGAACTCAGTGACACGCCGTTTAGCGTAGAGAAGGTGATTCAGGAACACATTG
ACGCCGGTATCACGCTGGCTAATGCCGTGAACTTTATTGTAGAGAAATACGAGCTGGTTCGTATCGATCGCAAGGGATTT
AGCTGGCAGGAACAATCACCCTATCTTCGGGCTGTAGACATTTTGAGGGCGCGACAGGCTACTGGCTTGATAAATCAGTG
CAAAAAACGCAAATGTAATCAGCCAGACGGTTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT194353 WP_000288812.1 NZ_CP070568:159734-159991 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT194353 NZ_CP093445:3731598-3731939 [Salmonella enterica subsp. enterica serovar Abeokuta]
ATGGACACAAATGAAACACAATCCACGCCAGTATGGGGACTACGGAACGATGTCTTCCCATCATTTGGCGCCCGGTTAGT
TCAGGAAGGTAATCGCCTGCACTATCTGGATGACCGGGCGGGTATTTACGGCGAATTTACCCCGGCGCAACTACAGACGC
TGGATATCGTATTTCCAATGTTTATTAAACACATGGAGGCCGCTTTACGCACTGGCGCACTCAACCCGCGTGAAGCCAGA
CGCTTTATGACTGAACTCCGGGGCATAACCTACGAAGCCGACACTCGCGGATCGTTCGGTTACGTTTATCTTTCGCTTTA
CCCAACCGTCGTTAAAAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0