Detailed information of TA system TA194351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 45283..45926
• Replicon: plasmid p51248_KPC
• Accession: NZ_CP070565
• Organism: Klebsiella pneumoniae strain KP51248

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: JW300_RS00260
• Protein ID: WP_001044770.1
• Coordinates: 45510..45926 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: JW300_RS00255
• Protein ID: WP_001261282.1
• Coordinates: 45283..45513 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW300_RS00220	40855..41721	-	867	WP_004118283.1	replication initiation protein	-
JW300_RS00225	42256..42360	+	105	WP_032409716.1	hypothetical protein	-
JW300_RS00230	42489..42746	+	258	WP_000764642.1	hypothetical protein	-
JW300_RS00235	42804..43580	-	777	WP_000015958.1	site-specific integrase	-
JW300_RS00240	43577..44320	-	744	WP_000129823.1	hypothetical protein	-
JW300_RS00245	44371..44721	-	351	WP_000493378.1	hypothetical protein	-
JW300_RS00250	44865..45326	-	462	WP_072202616.1	hypothetical protein	-
JW300_RS00255	45283..45513	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JW300_RS00260	45510..45926	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
JW300_RS00265	46000..46710	+	711	WP_004152334.1	ATP-binding protein	-
JW300_RS00270	46744..47441	+	698	Protein_53	IS1 family transposase	-
JW300_RS00275	47457..47567	+	111	Protein_54	mercuric transport protein periplasmic component	-
JW300_RS00280	47603..48025	+	423	WP_001340589.1	organomercurial transporter MerC	-
JW300_RS00285	48077..49771	+	1695	WP_000105636.1	mercury(II) reductase	-
JW300_RS00290	49789..50151	+	363	WP_001277456.1	mercury resistance co-regulator MerD	-
JW300_RS00295	50148..50384	+	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	armA / msr(E) / mph(E) / blaKPC-2 / blaTEM-1A / blaOXA-9	-	1..118664	118664

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T194351 WP_001044770.1 NZ_CP070565:45510-45926 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T194351 NZ_CP093445:2441559-2441843 [Salmonella enterica subsp. enterica serovar Abeokuta]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT194351 WP_001261282.1 NZ_CP070565:45283-45513 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT194351 NZ_CP093445:2441321-2441569 [Salmonella enterica subsp. enterica serovar Abeokuta]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTACCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3