Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5227183..5227808 Replicon chromosome
Accession NZ_CP070564
Organism Klebsiella pneumoniae strain KP51069

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JW299_RS27660 Protein ID WP_002882817.1
Coordinates 5227425..5227808 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JW299_RS27655 Protein ID WP_004150355.1
Coordinates 5227183..5227425 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW299_RS27630 5223174..5223773 + 600 WP_004151865.1 glucose-1-phosphatase -
JW299_RS27635 5223767..5224627 + 861 WP_002882807.1 virulence factor BrkB family protein -
JW299_RS27640 5224624..5225061 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JW299_RS27645 5225106..5226047 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JW299_RS27650 5226061..5226978 - 918 WP_002882812.1 alpha/beta hydrolase -
JW299_RS27655 5227183..5227425 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JW299_RS27660 5227425..5227808 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JW299_RS27665 5227982..5228911 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JW299_RS27670 5228908..5229543 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JW299_RS27675 5229540..5230442 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T194350 WP_002882817.1 NZ_CP070564:5227425-5227808 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T194350 NZ_CP093445:2071160-2071263 [Salmonella enterica subsp. enterica serovar Abeokuta]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT194350 WP_004150355.1 NZ_CP070564:5227183-5227425 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT194350 NZ_CP093445:c2071268-2071122 [Salmonella enterica subsp. enterica serovar Abeokuta]
CAAATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References