Detailed information of TA system TA194332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 63986..64722
• Replicon: plasmid p51069_IncFII
• Accession: NZ_CP070562
• Organism: Klebsiella pneumoniae strain KP51069

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: JW299_RS01245
• Protein ID: WP_003026803.1
• Coordinates: 64240..64722 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: JW299_RS01240
• Protein ID: WP_003026799.1
• Coordinates: 63986..64252 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW299_RS01195	60048..60410	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
JW299_RS01200	60460..60810	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
JW299_RS01205	61168..61437	+	270	WP_004152102.1	hypothetical protein	-
JW299_RS01210	61425..62000	+	576	WP_004152103.1	hypothetical protein	-
JW299_RS01215	62031..62525	+	495	WP_004152104.1	DNA-binding protein	-
JW299_RS01220	62569..62937	+	369	WP_004152105.1	hypothetical protein	-
JW299_RS01225	62971..63174	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
JW299_RS01230	63223..63480	+	258	WP_004152107.1	hypothetical protein	-
JW299_RS01235	63556..63810	+	255	WP_004152108.1	hypothetical protein	-
JW299_RS01240	63986..64252	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
JW299_RS01245	64240..64722	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
JW299_RS01250	64934..66280	+	1347	WP_020314316.1	ISNCY family transposase	-
JW299_RS01255	66781..68035	-	1255	Protein_66	IS3 family transposase	-
JW299_RS01260	68123..69085	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1	-	1..171286	171286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T194332 WP_003026803.1 NZ_CP070562:64240-64722 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T194332 NZ_CP093411:c4153019-4152711 [Salmonella enterica subsp. enterica serovar Agona]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGATGGGTTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT194332 WP_003026799.1 NZ_CP070562:63986-64252 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT194332 NZ_CP093411:c4153261-4153022 [Salmonella enterica subsp. enterica serovar Agona]
ATGTCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTAGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCTGCGGTGCTTACCGCTGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66