Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 61189..61832 Replicon plasmid p51069_KPC
Accession NZ_CP070560
Organism Klebsiella pneumoniae strain KP51069

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag JW299_RS00410 Protein ID WP_001044770.1
Coordinates 61416..61832 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag JW299_RS00405 Protein ID WP_001261282.1
Coordinates 61189..61419 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW299_RS00365 56387..56860 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
JW299_RS00370 56952..57182 + 231 WP_011977773.1 hypothetical protein -
JW299_RS00375 58074..58856 - 783 WP_004152340.1 site-specific integrase -
JW299_RS00380 58856..59188 - 333 WP_004152339.1 hypothetical protein -
JW299_RS00385 59195..59593 - 399 WP_004171440.1 hypothetical protein -
JW299_RS00390 59619..59948 - 330 WP_004152337.1 hypothetical protein -
JW299_RS00395 59976..60284 - 309 WP_004152336.1 hypothetical protein -
JW299_RS00400 60771..61232 - 462 WP_072093212.1 hypothetical protein -
JW299_RS00405 61189..61419 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JW299_RS00410 61416..61832 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
JW299_RS00415 61906..62616 + 711 WP_004152334.1 ATP-binding protein -
JW299_RS00420 62650..63347 + 698 Protein_83 IS1 family transposase -
JW299_RS00425 63363..63473 + 111 Protein_84 mercuric transport protein periplasmic component -
JW299_RS00430 63509..63931 + 423 WP_001340589.1 organomercurial transporter MerC -
JW299_RS00435 63983..65677 + 1695 WP_000105636.1 mercury(II) reductase -
JW299_RS00440 65695..66057 + 363 WP_001277456.1 mercury resistance co-regulator MerD -
JW299_RS00445 66054..66290 + 237 WP_000993386.1 broad-spectrum mercury transporter MerE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1A / blaOXA-9 / blaKPC-3 - 1..113456 113456
- flank IS/Tn - - 54948..56267 1319


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T194331 WP_001044770.1 NZ_CP070560:61416-61832 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T194331 NZ_CP093411:c4138046-4136718 [Salmonella enterica subsp. enterica serovar Agona]
ATGCGTCGAACACAGCAGCGTTTATCAATATGGATGAATGGAATCCGGGTCGGATTCTGGGAGAAGGCCAGAGGCGAGGA
TTTATTACAATACCTTCCAGAATGGATAATTGATGAACAGGGAAGACCTTTATCGCTTTCTTTGCCTTTCACTCCAGGTA
ATCAGCTTTGGCGTGGTAATGTTGTTCGTGACTATTTTGATAATTTATTGCCTGACAGCGAAAGTATACGCAGACGTTTA
GCCGTGCGTTACCAGGCTGAAAGCCTTGAGCCTTTTGATCTATTGGCTGAGCTGGGAAGAGACTGCGTTGGTGCAATACA
GTTACTGAATGTTGATGAAGAGCCCACAGATTTATTTTCCGTAAATTATCGCCCACTTTCTGAAGCTGATATCGCAACTA
CATTGCGTAATACTACGGCGATATCGTTGCCTGGTCGGCAGGACGAAACTGACGATTTGCGATTATCAATTGCCGGTGCG
CAGGAAAAAACGGCTTTATTGTGGCATGAAGAACGATGGTGTTTACCTGAAGGTAATACCCCAACAACGCATATCTTCAA
ACTACCGCTTGGGTTGGTTGGGAACATGCAAGCGGATATGAGTACATCGGTTGAAAATGAATGGCTGTGTTCTTTGCTTG
TTGAGCACTACGGGATCCCTGTAGCAAAAACACAGATTGCGCAGTTTGAGGATCAGAAGGCATTAGTAGTTGAGCGTTTC
GACAGAAGATGGTCAGGCGATCGGCAATGGATCATTCGTTTGCCACAAGAGGATATGTGTCAGGCTTTAGGTGTTTCTCC
GTTACGAAAATACCAGTCTGATGGTGGGCCGGGTATTTCCGATATTATGGAAATACTGAGTCATTCAGATCAGGCTGAGC
AGGACAAAGAGCAGTTCTTCAGGGCTCAAATTATTTTCTGGTTGATGGCTGCTACTGACGGCCATGCCAAAAATTTCAGT
ATCGCTATTGAGCCACAAAGTCGTTACCACCTTACGCCTCTTTACGATATTTTATCAGCATGGCCGGTAATTGGTCATGG
TAATAATCAGATTTCCTGGCAAAGATGCAAACTGGCAATGGCTGTTCGCGGTAGCAGTAATTATTACCACATTTATAGAG
TTCAACGACGGCATTGGATTAATCAAGGTGAATTAAACGGATTGGGAAGACGACAAGTTGAGTCCATGATGGATGACATT
ATATCCAGCACACCTGAAGTCATTGAGCGTGTATCTGCGTTGCTTCCAGAGTCGTTTCCATCTGAGCTTGCTGAGTGTAT
TTTTGAAGGTATGCGGCAGCAGTGTAGGCGTTTGGCTGGAAGGGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT194331 WP_001261282.1 NZ_CP070560:61189-61419 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT194331 NZ_CP093411:c4138356-4138051 [Salmonella enterica subsp. enterica serovar Agona]
ATGAATACTGTTTATCCGTTGAAAACTTTGAATCAAATTCGTCCATTGCTGATTGGCTTTCGCAAGGCTAATGGACTGAC
ACAAAAGGAAGTTTCTGAAAGGTTAGGAGTAACTCAGCAAACTTATGCCCGACTGGAAGCGAATCCAGCCAGTGCCAGTA
TGGAGCGACTATTCAGGGTATTTACCGTGCTGGGTGTAGAAATGGTACTCTCTTCTGGGCCACAGATACTTTCTTCGATG
TCATCTTCAACAATGGAAGAGTCAGAACAGCAAATAGATTCACCTGCCAGACGGGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References