Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 39509..40152 Replicon plasmid p50821_KPC
Accession NZ_CP070550
Organism Morganella morganii strain MM50821

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag JW297_RS00220 Protein ID WP_000754566.1
Coordinates 39509..39925 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag JW297_RS00225 Protein ID WP_001261276.1
Coordinates 39922..40152 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW297_RS00190 34686..34856 - 171 Protein_37 serine hydrolase -
JW297_RS00195 34911..35615 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
JW297_RS00200 35680..36204 + 525 Protein_39 Tn3 family transposase -
JW297_RS00205 36367..36450 - 84 Protein_40 SOS response-associated peptidase -
JW297_RS00210 37091..38167 - 1077 WP_000227969.1 IS110 family transposase -
JW297_RS00215 38449..39305 - 857 Protein_42 IS3-like element ISEc15 family transposase -
JW297_RS00220 39509..39925 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
JW297_RS00225 39922..40152 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JW297_RS00230 40726..41076 + 351 WP_000493378.1 hypothetical protein -
JW297_RS00235 41127..41870 + 744 WP_000129823.1 hypothetical protein -
JW297_RS00240 41867..42643 + 777 WP_000015958.1 site-specific integrase -
JW297_RS00245 42701..42958 - 258 WP_000764642.1 hypothetical protein -
JW297_RS00250 43087..43191 - 105 WP_032409716.1 hypothetical protein -
JW297_RS00255 43721..44587 + 867 WP_004118283.1 replication initiation protein -
JW297_RS00260 44764..45033 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid catB3 / ARR-3 / qacE / sul1 / mph(A) / blaKPC-2 / aac(6')-Ib - 1..54347 54347
- inside IScluster/Tn catB3 / ARR-3 / qacE / sul1 / mph(A) / blaKPC-2 / aac(6')-Ib - 355..39305 38950


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T194327 WP_000754566.1 NZ_CP070550:c39925-39509 [Morganella morganii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T194327 NZ_CP093411:2741881-2742192 [Salmonella enterica subsp. enterica serovar Agona]
ATGCATGTCATATCAAAAGAGCCTTTTGATGAAGCGGCAAGACGATATCCCAATGACTCGTTAGCCATAAAAGCCCTGTA
TCGCTTAGTCCGCGAAAAAGATTTCTCTTCTCCGGCAGAGCTACGAACGGTCATACCGAGCCTCGATAACTTTAAGTACC
GTAACAAGTGGTGGGTACTGGATGTCGGAGGCAACAACCTGAGGGTGATCGCTTACATCAACTTTATAAACAAACGCTTT
TATGTGAAACACATCGCCACTCACGCTGAATATGACAAGCTGACCCGCTATTACAGGGAGAATAAAGAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT194327 WP_001261276.1 NZ_CP070550:c40152-39922 [Morganella morganii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT194327 NZ_CP093411:2742189-2742608 [Salmonella enterica subsp. enterica serovar Agona]
ATGATTGCCAACACCGCAAAAGCTATCGAAGCCACAAAGGCGCTGGTCGCCGCCGTTCCTTTCCTGGGTGGCAGCGCGTC
AGAGAAAGATTATCGTGATGCGCTGGCGCTGGTTGATTATCTTATCGAAAATGACGATGAAAACCCGCTGATTGACTTCC
TGGCAAGCAAAATTGCCGAGTACGAAGATAACAGCAAGCAGTTCGCCGAGTTCAACAAATCTGTTGCTGAAATGCCGGTT
GGCGTTGCCCTGCTGCGCACGCTAATTGACCAGTACAAACTCTCATACTCAGATCTTAAAGAAGAAATCGGTTCCAAATC
ACTGGTAAGTCAGATCCTGTCCGGACAGCGCTCGTTAACGATTACGCACATCAAAGCGCTTTCAGCACGATTTGGCGTGA
AACCTGAGTGGTTCTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References