Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4773937..4774557 Replicon chromosome
Accession NZ_CP070549
Organism Citrobacter freundii strain CF49969

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag JW296_RS25390 Protein ID WP_002892050.1
Coordinates 4773937..4774155 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag JW296_RS25395 Protein ID WP_003021733.1
Coordinates 4774183..4774557 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW296_RS25355 4769170..4769799 + 630 WP_003835929.1 membrane protein -
JW296_RS25360 4769864..4770217 + 354 WP_003831033.1 DUF1428 family protein -
JW296_RS25365 4770269..4771822 - 1554 WP_003835927.1 EAL domain-containing protein -
JW296_RS25370 4772011..4772271 + 261 WP_003021719.1 type B 50S ribosomal protein L31 -
JW296_RS25375 4772273..4772413 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
JW296_RS25380 4772492..4772962 - 471 WP_087879033.1 YlaC family protein -
JW296_RS25385 4773079..4773630 - 552 WP_003835924.1 maltose O-acetyltransferase -
JW296_RS25390 4773937..4774155 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
JW296_RS25395 4774183..4774557 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
JW296_RS25400 4775046..4778195 - 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
JW296_RS25405 4778218..4779411 - 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T194325 WP_002892050.1 NZ_CP070549:c4774155-4773937 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T194325 NZ_CP093411:c1028416-1027889 [Salmonella enterica subsp. enterica serovar Agona]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGAATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACAGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGATCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT194325 WP_003021733.1 NZ_CP070549:c4774557-4774183 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT194325 NZ_CP093411:c1028703-1028413 [Salmonella enterica subsp. enterica serovar Agona]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References