Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4110008..4110524 Replicon chromosome
Accession NZ_CP070549
Organism Citrobacter freundii strain CF49969

Toxin (Protein)


Gene name relE Uniprot ID A0A133L8K5
Locus tag JW296_RS22330 Protein ID WP_003844927.1
Coordinates 4110240..4110524 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0J1MV10
Locus tag JW296_RS22325 Protein ID WP_003839576.1
Coordinates 4110008..4110250 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW296_RS22305 4105023..4106156 + 1134 WP_060855415.1 amidohydrolase/deacetylase family metallohydrolase -
JW296_RS22310 4106140..4107258 + 1119 WP_054527995.1 DgaE family pyridoxal phosphate-dependent ammonia lyase -
JW296_RS22315 4107255..4107995 + 741 WP_048216922.1 KDGP aldolase family protein -
JW296_RS22320 4108017..4109930 + 1914 WP_003844930.1 BglG family transcription antiterminator -
JW296_RS22325 4110008..4110250 + 243 WP_003839576.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
JW296_RS22330 4110240..4110524 + 285 WP_003844927.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JW296_RS22335 4110528..4110992 - 465 WP_032937736.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
JW296_RS22340 4111099..4113237 - 2139 WP_003844922.1 anaerobic ribonucleoside-triphosphate reductase -
JW296_RS22345 4113646..4115298 - 1653 WP_205461297.1 alpha,alpha-phosphotrehalase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-82)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-69)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10892.76 Da        Isoelectric Point: 10.0482

>T194324 WP_003844927.1 NZ_CP070549:4110240-4110524 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQFKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLLYQVRDEVVIVFVVAVGK
REHSAVYLDANKRL

Download         Length: 285 bp

>T194324 NZ_CP093411:842329-842742 [Salmonella enterica subsp. enterica serovar Agona]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8961.34 Da        Isoelectric Point: 5.0651

>AT194324 WP_003839576.1 NZ_CP070549:4110008-4110250 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDTLIQTVRERLASPQKGIKVSLDDL

Download         Length: 243 bp

>AT194324 NZ_CP093411:842082-842348 [Salmonella enterica subsp. enterica serovar Agona]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133L8K5

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1MV10

References