Detailed information of TA system TA194318

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2666213..2666968
• Replicon: chromosome
• Accession: NZ_CP070549
• Organism: Citrobacter freundii strain CF49969

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7L6ILI8
• Locus tag: JW296_RS15330
• Protein ID: WP_047359063.1
• Coordinates: 2666591..2666968 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JW296_RS15325
• Protein ID: WP_162906152.1
• Coordinates: 2666213..2666539 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW296_RS15285	2661437..2662258	+	822	WP_032751326.1	DUF945 domain-containing protein	-
JW296_RS15290	2662474..2663175	+	702	WP_047359069.1	WYL domain-containing protein	-
JW296_RS15295	2663358..2664053	+	696	WP_047359068.1	hypothetical protein	-
JW296_RS15300	2664075..2664548	+	474	WP_052951289.1	hypothetical protein	-
JW296_RS15305	2664626..2664865	+	240	WP_047359067.1	DUF905 domain-containing protein	-
JW296_RS15310	2664962..2665423	+	462	WP_047359066.1	antirestriction protein	-
JW296_RS15315	2665435..2665914	+	480	WP_047359065.1	DNA repair protein RadC	-
JW296_RS15320	2665935..2666156	+	222	WP_000691982.1	DUF987 domain-containing protein	-
JW296_RS15325	2666213..2666539	+	327	WP_162906152.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JW296_RS15330	2666591..2666968	+	378	WP_047359063.1	TA system toxin CbtA family protein	Toxin
JW296_RS15335	2666965..2667456	+	492	WP_047359062.1	hypothetical protein	-
JW296_RS15340	2667485..2667688	+	204	WP_047359061.1	DUF957 domain-containing protein	-
JW296_RS15345	2667767..2668615	+	849	WP_088732394.1	DUF4942 domain-containing protein	-
JW296_RS15350	2669087..2670946	-	1860	WP_003838200.1	bifunctional glutathionylspermidine amidase/synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-110)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14078.86 Da
• Isoelectric Point: 7.0606

>T194318 WP_047359063.1 NZ_CP070549:2666591-2666968 [Citrobacter freundii]
VQTQPLSSTHETSPRPSPVDIWQHLLNHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
SADTQSPLIGSIDILRARKATGLMTRHGYRPVTDLITGKYKKEQQ

Nucleotide

• Length: 378 bp

>T194318 NZ_CP093407:c4180453-4180145 [Salmonella enterica subsp. enterica serovar Agona]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGATGGGTTCTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11955.62 Da
• Isoelectric Point: 6.9554

>AT194318 WP_162906152.1 NZ_CP070549:2666213-2666539 [Citrobacter freundii]
ITEPWWGLKRSITPCFGARLVQEGNRLHYLGDRASIAGTFSDADLRHLDQAFPVQLKQLELMLVSGELNPRHQHCVTLYS
KGLTCEADSLGSHGYVYIAIYPTPAATA

Nucleotide

• Length: 327 bp

>AT194318 NZ_CP093407:c4180695-4180456 [Salmonella enterica subsp. enterica serovar Agona]
ATGTCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTAGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCTGCGGTGCTTACCGCTGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Structures

Toxin

• AlphaFold DB: A0A7L6ILI8