Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 244861..245664 Replicon plasmid p49969_KPC
Accession NZ_CP070545
Organism Citrobacter freundii strain CF49969

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag JW296_RS01460 Protein ID WP_022652313.1
Coordinates 245134..245664 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag JW296_RS01455 Protein ID WP_022652312.1
Coordinates 244861..245130 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW296_RS01440 241166..241473 - 308 Protein_287 IS1 family transposase -
JW296_RS01445 241950..242690 + 741 WP_022652310.1 site-specific integrase -
JW296_RS01450 243016..244005 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
JW296_RS01455 244861..245130 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
JW296_RS01460 245134..245664 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
JW296_RS01465 245796..246536 + 741 Protein_292 IS5 family transposase -
JW296_RS01470 246594..247358 - 765 WP_071444478.1 IS21-like element helper ATPase IstB -
JW296_RS01475 247355..248854 - 1500 WP_071444479.1 IS21 family transposase -
JW296_RS01480 248947..249105 + 159 Protein_295 transposase -
JW296_RS01485 249246..250142 - 897 Protein_296 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaTEM-1B / aac(3)-IId / aph(2'')-Ib / aac(6')-Im - 1..269326 269326
- inside IScluster/Tn - - 239852..260389 20537


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T194306 WP_022652313.1 NZ_CP070545:245134-245664 [Citrobacter freundii]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T194306 NZ_CP093405:c4733203-4732892 [Salmonella enterica subsp. enterica serovar Agona]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT194306 WP_022652312.1 NZ_CP070545:244861-245130 [Citrobacter freundii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT194306 NZ_CP093405:c4732891-4732601 [Salmonella enterica subsp. enterica serovar Agona]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGGTTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGGTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References