Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 102370..103013 Replicon plasmid p49969_KPC
Accession NZ_CP070545
Organism Citrobacter freundii strain CF49969

Toxin (Protein)


Gene name vagD Uniprot ID A0A6C0NE46
Locus tag JW296_RS00565 Protein ID WP_008322233.1
Coordinates 102597..103013 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag JW296_RS00560 Protein ID WP_001261276.1
Coordinates 102370..102600 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW296_RS00530 97915..98211 - 297 WP_081364705.1 type II toxin-antitoxin system RelE/ParE family toxin -
JW296_RS00535 98198..98458 - 261 WP_040219223.1 type II toxin-antitoxin system ParD family antitoxin -
JW296_RS00540 98831..99061 + 231 WP_008322219.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JW296_RS00545 99058..99474 + 417 WP_008322222.1 type II toxin-antitoxin system VapC family toxin -
JW296_RS00550 99548..101110 + 1563 WP_008322224.1 AAA family ATPase -
JW296_RS00555 101095..102117 + 1023 WP_008322227.1 helicase UvrD -
JW296_RS00560 102370..102600 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JW296_RS00565 102597..103013 + 417 WP_008322233.1 type II toxin-antitoxin system VapC family toxin Toxin
JW296_RS00570 103207..103376 + 170 Protein_113 DUF596 domain-containing protein -
JW296_RS00575 103559..104479 - 921 WP_008322235.1 carbohydrate kinase -
JW296_RS00580 104476..105846 - 1371 WP_008322237.1 hypothetical protein -
JW296_RS00585 105924..106943 - 1020 WP_004113181.1 ribose ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaTEM-1B / aac(3)-IId / aph(2'')-Ib / aac(6')-Im - 1..269326 269326


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15054.58 Da        Isoelectric Point: 9.2957

>T194304 WP_008322233.1 NZ_CP070545:102597-103013 [Citrobacter freundii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAASAVLVTNNVREFARVPGLMLEDWVK

Download         Length: 417 bp

>T194304 NZ_CP093405:c4197987-4197679 [Salmonella enterica subsp. enterica serovar Agona]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGATGGGTTCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT194304 WP_001261276.1 NZ_CP070545:102370-102600 [Citrobacter freundii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT194304 NZ_CP093405:c4198229-4197990 [Salmonella enterica subsp. enterica serovar Agona]
ATGTCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTAGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCTGCGGTGCTTACCGCTGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE46

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References