Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 98831..99474 Replicon plasmid p49969_KPC
Accession NZ_CP070545
Organism Citrobacter freundii strain CF49969

Toxin (Protein)


Gene name vagD Uniprot ID A0A6C0NGR2
Locus tag JW296_RS00545 Protein ID WP_008322222.1
Coordinates 99058..99474 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A6C0NE67
Locus tag JW296_RS00540 Protein ID WP_008322219.1
Coordinates 98831..99061 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW296_RS00515 95648..96352 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
JW296_RS00520 96382..97032 + 651 Protein_103 copper resistance membrane spanning protein PcoS -
JW296_RS00525 97249..97683 + 435 WP_004152085.1 copper resistance system metallochaperone PcoE -
JW296_RS00530 97915..98211 - 297 WP_081364705.1 type II toxin-antitoxin system RelE/ParE family toxin -
JW296_RS00535 98198..98458 - 261 WP_040219223.1 type II toxin-antitoxin system ParD family antitoxin -
JW296_RS00540 98831..99061 + 231 WP_008322219.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JW296_RS00545 99058..99474 + 417 WP_008322222.1 type II toxin-antitoxin system VapC family toxin Toxin
JW296_RS00550 99548..101110 + 1563 WP_008322224.1 AAA family ATPase -
JW296_RS00555 101095..102117 + 1023 WP_008322227.1 helicase UvrD -
JW296_RS00560 102370..102600 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JW296_RS00565 102597..103013 + 417 WP_008322233.1 type II toxin-antitoxin system VapC family toxin -
JW296_RS00570 103207..103376 + 170 Protein_113 DUF596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaTEM-1B / aac(3)-IId / aph(2'')-Ib / aac(6')-Im - 1..269326 269326


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15188.56 Da        Isoelectric Point: 7.1361

>T194303 WP_008322222.1 NZ_CP070545:99058-99474 [Citrobacter freundii]
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRNQRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T194303 NZ_CP093405:c4183014-4181686 [Salmonella enterica subsp. enterica serovar Agona]
ATGCGTCGAACACAGCAGCGTTTATCAATATGGATGAATGGAATCCGGGTCGGATTCTGGGAGAAGGCCAGAGGCGAGGA
TTTATTACAATACCTTCCAGAATGGATAATTGATGAACAGGGAAGACCTTTATCGCTTTCTTTGCCTTTCACTCCAGGTA
ATCAGCTTTGGCGTGGTAATGTTGTTCGTGACTATTTTGATAATTTATTGCCTGACAGCGAAAGTATACGCAGACGTTTA
GCCGTGCGTTACCAGGCTGAAAGCCTTGAGCCTTTTGATCTATTGGCTGAGCTGGGAAGAGACTGCGTTGGTGCAATACA
GTTACTGAATGTTGATGAAGAGCCCACAGATTTATTTTCCGTAAATTATCGCCCACTTTCTGAAGCTGATATCGCAACTA
CATTGCGTAATACTACGGCGATATCGTTGCCTGGTCGGCAGGACGAAACTGACGATTTGCGATTATCAATTGCCGGTGCG
CAGGAAAAAACGGCTTTATTGTGGCATGAAGAACGATGGTGTTTACCTGAAGGTAATACCCCAACAACGCATATCTTCAA
ACTACCGCTTGGGTTGGTTGGGAACATGCAAGCGGATATGAGTACATCGGTTGAAAATGAATGGCTGTGTTCTTTGCTTG
TTGAGCACTACGGGATCCCTGTAGCAAAAACACAGATTGCGCAGTTTGAGGATCAGAAGGCATTAGTAGTTGAGCGTTTC
GACAGAAGATGGTCAGGCGATCGGCAATGGATCATTCGTTTGCCACAAGAGGATATGTGTCAGGCTTTAGGTGTTTCTCC
GTTACGAAAATACCAGTCTGATGGTGGGCCGGGTATTTCCGATATTATGGAAATACTGAGTCATTCAGATCAGGCTGAGC
AGGACAAAGAGCAGTTCTTCAGGGCTCAAATTATTTTCTGGTTGATGGCTGCTACTGACGGCCATGCCAAAAATTTCAGT
ATCGCTATTGAGCCACAAAGTCGTTACCACCTTACGCCTCTTTACGATATTTTATCAGCATGGCCGGTAATTGGTCATGG
TAATAATCAGATTTCCTGGCAAAGATGCAAACTGGCAATGGCTGTTCGCGGTAGCAGTAATTATTACCACATTTATAGAG
TTCAACGACGGCATTGGATTAATCAAGGTGAATTAAACGGATTGGGAAGACGACAAGTTGAGTCCATGATGGATGACATT
ATATCCAGCACACCTGAAGTCATTGAGCGTGTATCTGCGTTGCTTCCAGAGTCGTTTCCATCTGAGCTTGCTGAGTGTAT
TTTTGAAGGTATGCGGCAGCAGTGTAGGCGTTTGGCTGGAAGGGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8736.84 Da        Isoelectric Point: 4.4692

>AT194303 WP_008322219.1 NZ_CP070545:98831-99061 [Citrobacter freundii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFNL

Download         Length: 231 bp

>AT194303 NZ_CP093405:c4183324-4183019 [Salmonella enterica subsp. enterica serovar Agona]
ATGAATACTGTTTATCCGTTGAAAACTTTGAATCAAATTCGTCCATTGCTGATTGGCTTTCGCAAGGCTAATGGACTGAC
ACAAAAGGAAGTTTCTGAAAGGTTAGGAGTAACTCAGCAAACTTATGCCCGACTGGAAGCGAATCCAGCCAGTGCCAGTA
TGGAGCGACTATTCAGGGTATTTACCGTGCTGGGTGTAGAAATGGTACTCTCTTCTGGGCCACAGATACTTTCTTCGATG
TCATCTTCAACAATGGAAGAGTCAGAACAGCAAATAGATTCACCTGCCAGACGGGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NGR2

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE67

References