Detailed information of TA system TA194301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1976679..1977496
• Replicon: chromosome
• Accession: NZ_CP070537
• Organism: Morganella morganii strain MM48659

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A0A2C5TM43
• Locus tag: JW294_RS09930
• Protein ID: WP_004236532.1
• Coordinates: 1976679..1977161 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: JW294_RS09935
• Protein ID: WP_032097998.1
• Coordinates: 1977188..1977496 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW294_RS09895	1972038..1972661	-	624	WP_004236525.1	thiol:disulfide interchange protein DsbA	-
JW294_RS09900	1972680..1973675	-	996	WP_004236526.1	serine/threonine protein kinase	-
JW294_RS09905	1973675..1973944	-	270	WP_036415677.1	YihD family protein	-
JW294_RS09910	1974147..1975262	+	1116	WP_205458409.1	molybdenum cofactor guanylyltransferase MobA	-
JW294_RS09915	1975477..1975779	+	303	WP_004240743.1	exported protein precursor	-
JW294_RS09920	1975907..1976428	+	522	WP_015422407.1	isopentenyl-diphosphate Delta-isomerase	-
JW294_RS09925	1976425..1976565	+	141	WP_004236531.1	hypothetical protein	-
JW294_RS09930	1976679..1977161	-	483	WP_004236532.1	GNAT family N-acetyltransferase	Toxin
JW294_RS09935	1977188..1977496	-	309	WP_032097998.1	DUF1778 domain-containing protein	Antitoxin
JW294_RS09940	1977628..1978980	-	1353	WP_032098064.1	glutathione-disulfide reductase	-
JW294_RS09945	1979049..1979891	-	843	WP_004236535.1	23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ	-
JW294_RS09950	1980081..1980470	+	390	WP_004236536.1	DUF1090 domain-containing protein	-
JW294_RS09955	1980882..1981874	+	993	WP_032098000.1	inner membrane protein YhjD	-
JW294_RS09960	1981982..1982188	+	207	WP_004236540.1	4-oxalocrotonate tautomerase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (36-135)

Antitoxin

• Vibrio phage ICP1, Orf50 (21-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17729.48 Da
• Isoelectric Point: 10.2370

>T194301 WP_004236532.1 NZ_CP070537:c1977161-1976679 [Morganella morganii]
MQQHHDFTVFQSGEESLNHWLKTRALQNQQSGASRTFVVCHHNRIMAYYALSTGVITCSQAAGRFRRNMPPEIPVILPGR
LAVDESVKGRGIGRGLIKDAALRVLQAAGIVGIRGIVVRALSDNARRFYEHTGFMPSPADPMLLMITLRDLQLATGIYSE

Nucleotide

• Length: 483 bp

>T194301 NZ_CP093405:3516049-3516267 [Salmonella enterica subsp. enterica serovar Agona]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11469.12 Da
• Isoelectric Point: 8.6397

>AT194301 WP_032097998.1 NZ_CP070537:c1977496-1977188 [Morganella morganii]
MTKHTDEQHPVSGVNITRSTLNLRIKPEDKILIDRAAHAAGKNRTEFVLEAARRAAEETLADLRVIDVSTDVYQQFITRL
DMAPQSGEALKKTMLAKAPWEK

Nucleotide

• Length: 309 bp

>AT194301 NZ_CP093405:3515647-3516021 [Salmonella enterica subsp. enterica serovar Agona]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A2C5TM43