Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 2561106..2561903 Replicon chromosome
Accession NZ_CP070534
Organism Enterobacter hormaechei strain EC48293

Toxin (Protein)


Gene name KacT Uniprot ID A0A222RDD1
Locus tag JW293_RS14250 Protein ID WP_032647926.1
Coordinates 2561382..2561903 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A331QJY7
Locus tag JW293_RS14245 Protein ID WP_015570876.1
Coordinates 2561106..2561375 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW293_RS14210 2556646..2557005 + 360 WP_032647921.1 helix-turn-helix transcriptional regulator -
JW293_RS14215 2557015..2557179 - 165 WP_017693668.1 helix-turn-helix transcriptional regulator -
JW293_RS14220 2557332..2558573 + 1242 WP_045324885.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
JW293_RS14225 2558606..2558833 - 228 WP_006809324.1 YccJ family protein -
JW293_RS14230 2558852..2559448 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
JW293_RS14235 2559840..2560010 + 171 WP_001273664.1 general stress protein -
JW293_RS14240 2560127..2561032 + 906 WP_017693666.1 DMT family transporter -
JW293_RS14245 2561106..2561375 + 270 WP_015570876.1 DUF1778 domain-containing protein Antitoxin
JW293_RS14250 2561382..2561903 + 522 WP_032647926.1 GNAT family N-acetyltransferase Toxin
JW293_RS14255 2561962..2563284 - 1323 WP_032647928.1 pyrimidine utilization transport protein G -
JW293_RS14260 2563306..2563797 - 492 WP_032647931.1 pyrimidine utilization flavin reductase protein F -
JW293_RS14265 2563810..2564400 - 591 WP_032647932.1 malonic semialdehyde reductase -
JW293_RS14270 2564410..2565210 - 801 WP_032647935.1 pyrimidine utilization protein D -
JW293_RS14275 2565218..2565604 - 387 WP_015570870.1 pyrimidine utilization protein C -
JW293_RS14280 2565616..2566305 - 690 WP_017384771.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19451.21 Da        Isoelectric Point: 7.4318

>T194288 WP_032647926.1 NZ_CP070534:2561382-2561903 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLIRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T194288 NZ_CP093403:3502285-3502503 [Salmonella enterica subsp. enterica serovar Agona]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10247.75 Da        Isoelectric Point: 5.8836

>AT194288 WP_015570876.1 NZ_CP070534:2561106-2561375 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWIAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT194288 NZ_CP093403:3501883-3502257 [Salmonella enterica subsp. enterica serovar Agona]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A222RDD1

Antitoxin

Source ID Structure
AlphaFold DB A0A331QJY7

References