Detailed information of TA system TA194286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1914878..1915498
• Replicon: chromosome
• Accession: NZ_CP070534
• Organism: Enterobacter hormaechei strain EC48293

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A837FFM2
• Locus tag: JW293_RS11155
• Protein ID: WP_015571250.1
• Coordinates: 1914878..1915096 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: JW293_RS11160
• Protein ID: WP_204622408.1
• Coordinates: 1915124..1915498 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW293_RS11125	1910890..1911150	+	261	WP_015571255.1	type B 50S ribosomal protein L31	-
JW293_RS11130	1911153..1911293	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
JW293_RS11135	1911290..1912000	-	711	WP_032647453.1	GNAT family N-acetyltransferase	-
JW293_RS11140	1912102..1913562	+	1461	WP_032647455.1	PLP-dependent aminotransferase family protein	-
JW293_RS11145	1913534..1914001	-	468	WP_015571252.1	extracytoplasmic function sigma factor YlaC	-
JW293_RS11150	1914118..1914669	-	552	WP_032647457.1	maltose O-acetyltransferase	-
JW293_RS11155	1914878..1915096	-	219	WP_015571250.1	hemolysin expression modulator Hha	Toxin
JW293_RS11160	1915124..1915498	-	375	WP_204622408.1	Hha toxicity modulator TomB	Antitoxin
JW293_RS11165	1916009..1919155	-	3147	WP_015571248.1	multidrug efflux RND transporter permease subunit	-
JW293_RS11170	1919178..1920371	-	1194	WP_017694395.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8583.95 Da
• Isoelectric Point: 8.9008

>T194286 WP_015571250.1 NZ_CP070534:c1915096-1914878 [Enterobacter hormaechei]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 219 bp

>T194286 NZ_CP093403:2769740-2770051 [Salmonella enterica subsp. enterica serovar Agona]
ATGCATGTCATATCAAAAGAGCCTTTTGATGAAGCGGCAAGACGATATCCCAATGACTCGTTAGCCATAAAAGCCCTGTA
TCGCTTAGTCCGCGAAAAAGATTTCTCTTCTCCGGCAGAGCTACGAACGGTCATACCGAGCCTCGATAACTTTAAGTACC
GTAACAAGTGGTGGGTACTGGATGTCGGAGGCAACAACCTGAGGGTGATCGCTTACATCAACTTTATAAACAAACGCTTT
TATGTGAAACACATCGCCACTCACGCTGAATATGACAAGCTGACCCGCTATTACAGGGAGAATAAAGAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14478.23 Da
• Isoelectric Point: 4.8936

>AT194286 WP_204622408.1 NZ_CP070534:c1915498-1915124 [Enterobacter hormaechei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWHKSGNRLFRCFTNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT194286 NZ_CP093403:2770048-2770467 [Salmonella enterica subsp. enterica serovar Agona]
ATGATTGCCAACACCGCAAAAGCTATCGAAGCCACAAAGGCGCTGGTCGCCGCCGTTCCTTTCCTGGGTGGCAGCGCGTC
AGAGAAAGATTATCGTGATGCGCTGGCGCTGGTTGATTATCTTATCGAAAATGACGATGAAAACCCGCTGATTGACTTCC
TGGCAAGCAAAATTGCCGAGTACGAAGATAACAGCAAGCAGTTCGCCGAGTTCAACAAATCTGTTGCTGAAATGCCGGTT
GGCGTTGCCCTGCTGCGCACGCTAATTGACCAGTACAAACTCTCATACTCAGATCTTAAAGAAGAAATCGGTTCCAAATC
ACTGGTAAGTCAGATCCTGTCCGGACAGCGCTCGTTAACGATTACGCACATCAAAGCGCTTTCAGCACGATTTGGCGTGA
AACCTGAGTGGTTCTTATAA

Structures

Toxin

• AlphaFold DB: A0A837FFM2