Detailed information of TA system TA194285

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1765516..1766231
• Replicon: chromosome
• Accession: NZ_CP070534
• Organism: Enterobacter hormaechei strain EC48293

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8I0UM05
• Locus tag: JW293_RS10445
• Protein ID: WP_023303149.1
• Coordinates: 1765863..1766231 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8I0UJV2
• Locus tag: JW293_RS10440
• Protein ID: WP_023303148.1
• Coordinates: 1765516..1765842 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW293_RS10415	1760550..1761815	+	1266	WP_045324920.1	hypothetical protein	-
JW293_RS10420	1762124..1762558	+	435	WP_023303144.1	hypothetical protein	-
JW293_RS10425	1762617..1763456	+	840	WP_023303145.1	hypothetical protein	-
JW293_RS10430	1764146..1764967	+	822	WP_023303146.1	DUF945 domain-containing protein	-
JW293_RS10435	1765033..1765503	+	471	WP_023303147.1	DNA repair protein RadC	-
JW293_RS10440	1765516..1765842	+	327	WP_023303148.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JW293_RS10445	1765863..1766231	+	369	WP_023303149.1	TA system toxin CbtA family protein	Toxin
JW293_RS10450	1766581..1767327	-	747	WP_032647364.1	class I SAM-dependent methyltransferase	-
JW293_RS10455	1767338..1767595	-	258	WP_003863178.1	YjhX family toxin	-
JW293_RS10460	1767855..1769549	-	1695	Protein_1605	bifunctional diguanylate cyclase/phosphodiesterase	-
JW293_RS10465	1769875..1770861	+	987	WP_058671381.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1744598..1780642	36044

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13727.91 Da
• Isoelectric Point: 8.2822

>T194285 WP_023303149.1 NZ_CP070534:1765863-1766231 [Enterobacter hormaechei]
MKNLPATISRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGIILADAVNFLVDKYELVRIDRRGF
SSQGQVPYLTVTDILHARRACGLMKSCSYREVSNIVLSHSRQ

Nucleotide

• Length: 369 bp

>T194285 NZ_CP093403:c2426276-2425992 [Salmonella enterica subsp. enterica serovar Agona]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACTATACAGGAACAGTTTAA
AAAAAAGTTACGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12215.04 Da
• Isoelectric Point: 7.0266

>AT194285 WP_023303148.1 NZ_CP070534:1765516-1765842 [Enterobacter hormaechei]
MSLIPAHEWGIKSDIVPRLGVRLVQEGNRLHYLADRASITGKFSDAECLKLDIVFPHFIRQMESMLTTGEMNSRHAHCVT
LYHNGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 327 bp

>AT194285 NZ_CP093403:c2426514-2426266 [Salmonella enterica subsp. enterica serovar Agona]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA