Detailed information of TA system TA194284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 1410117..1410693
• Replicon: chromosome
• Accession: NZ_CP070534
• Organism: Enterobacter hormaechei strain EC48293

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A800YKM1
• Locus tag: JW293_RS08775
• Protein ID: WP_015572580.1
• Coordinates: 1410117..1410404 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A801DSF4
• Locus tag: JW293_RS08780
• Protein ID: WP_017694570.1
• Coordinates: 1410391..1410693 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW293_RS08750	1405235..1405930	+	696	WP_032647138.1	winged helix-turn-helix domain-containing protein	-
JW293_RS08755	1406080..1406550	+	471	WP_032647140.1	MarR family transcriptional regulator	-
JW293_RS08760	1406547..1407614	+	1068	WP_026094404.1	HlyD family secretion protein	-
JW293_RS08765	1407604..1408680	+	1077	WP_015572578.1	DUF2955 domain-containing protein	-
JW293_RS08770	1408677..1409948	-	1272	WP_032647142.1	DUF445 domain-containing protein	-
JW293_RS08775	1410117..1410404	+	288	WP_015572580.1	BrnT family toxin	Toxin
JW293_RS08780	1410391..1410693	+	303	WP_017694570.1	BrnA antitoxin family protein	Antitoxin
JW293_RS08785	1410722..1411360	-	639	WP_032647144.1	LysE family translocator	-
JW293_RS08790	1411399..1412151	-	753	WP_015572582.1	AraC family transcriptional regulator	-
JW293_RS08795	1412305..1413675	+	1371	WP_032647147.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
JW293_RS08800	1413853..1414398	-	546	WP_006810254.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11296.81 Da
• Isoelectric Point: 7.3587

>T194284 WP_015572580.1 NZ_CP070534:1410117-1410404 [Enterobacter hormaechei]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIRIIS
ARKADRKERRRYEHR

Nucleotide

• Length: 288 bp

>T194284 NZ_CP093403:c1056275-1055748 [Salmonella enterica subsp. enterica serovar Agona]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGAATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACAGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGATCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11482.13 Da
• Isoelectric Point: 10.4094

>AT194284 WP_017694570.1 NZ_CP070534:1410391-1410693 [Enterobacter hormaechei]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT194284 NZ_CP093403:c1056562-1056272 [Salmonella enterica subsp. enterica serovar Agona]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA