Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5266355..5266980 Replicon chromosome
Accession NZ_CP070528
Organism Klebsiella pneumoniae strain KP47693

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JW292_RS26280 Protein ID WP_002882817.1
Coordinates 5266355..5266738 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JW292_RS26285 Protein ID WP_004150355.1
Coordinates 5266738..5266980 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW292_RS26265 5263721..5264623 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JW292_RS26270 5264620..5265255 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JW292_RS26275 5265252..5266181 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JW292_RS26280 5266355..5266738 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JW292_RS26285 5266738..5266980 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JW292_RS26290 5267185..5268102 + 918 WP_032418237.1 alpha/beta hydrolase -
JW292_RS26295 5268116..5269057 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JW292_RS26300 5269102..5269539 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JW292_RS26305 5269536..5270396 - 861 WP_004146232.1 virulence factor BrkB family protein -
JW292_RS26310 5270390..5270989 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T194280 WP_002882817.1 NZ_CP070528:c5266738-5266355 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T194280 NZ_CP070528:c5266738-5266355 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT194280 WP_004150355.1 NZ_CP070528:c5266980-5266738 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT194280 NZ_CP070528:c5266980-5266738 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References