Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4667594..4668404 Replicon chromosome
Accession NZ_CP070528
Organism Klebsiella pneumoniae strain KP47693

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag JW292_RS23455 Protein ID WP_004178461.1
Coordinates 4667594..4668127 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JW292_RS23460 Protein ID WP_002887278.1
Coordinates 4668138..4668404 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW292_RS23450 4666425..4667546 + 1122 WP_004214138.1 cupin domain-containing protein -
JW292_RS23455 4667594..4668127 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
JW292_RS23460 4668138..4668404 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JW292_RS23465 4668507..4669940 - 1434 WP_032418099.1 Cu(+)/Ag(+) sensor histidine kinase -
JW292_RS23470 4669930..4670613 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
JW292_RS23475 4670785..4672170 + 1386 WP_032418101.1 efflux transporter outer membrane subunit -
JW292_RS23480 4672188..4672532 + 345 WP_032418103.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T194278 WP_004178461.1 NZ_CP070528:c4668127-4667594 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T194278 NZ_CP093402:c4275678-4275370 [Salmonella enterica subsp. enterica serovar Agona]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGATGGGTTCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT194278 WP_002887278.1 NZ_CP070528:c4668404-4668138 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT194278 NZ_CP093402:c4275920-4275681 [Salmonella enterica subsp. enterica serovar Agona]
ATGTCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTAGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCTGCGGTGCTTACCGCTGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References