Detailed information of TA system TA194263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 63964..64607
• Replicon: plasmid p47693_KPC
• Accession: NZ_CP070525
• Organism: Klebsiella pneumoniae strain KP47693

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: JW292_RS00335
• Protein ID: WP_001044770.1
• Coordinates: 63964..64380 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: JW292_RS00340
• Protein ID: WP_001261282.1
• Coordinates: 64377..64607 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW292_RS00300	59506..59742	-	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-
JW292_RS00305	59739..60101	-	363	WP_001277456.1	mercury resistance co-regulator MerD	-
JW292_RS00310	60119..61813	-	1695	WP_000105636.1	mercury(II) reductase	-
JW292_RS00315	61865..62287	-	423	WP_001340589.1	organomercurial transporter MerC	-
JW292_RS00320	62323..62433	-	111	Protein_63	mercuric transport protein periplasmic component	-
JW292_RS00325	62449..63146	-	698	Protein_64	IS1 family transposase	-
JW292_RS00330	63180..63890	-	711	WP_004152334.1	ATP-binding protein	-
JW292_RS00335	63964..64380	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
JW292_RS00340	64377..64607	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JW292_RS00345	64564..65025	+	462	WP_072202616.1	hypothetical protein	-
JW292_RS00350	65169..65519	+	351	WP_000493378.1	hypothetical protein	-
JW292_RS00355	65570..66313	+	744	WP_000129823.1	hypothetical protein	-
JW292_RS00360	66310..67086	+	777	WP_000015958.1	site-specific integrase	-
JW292_RS00365	67144..67401	-	258	WP_000764642.1	hypothetical protein	-
JW292_RS00370	67530..67634	-	105	WP_032409716.1	hypothetical protein	-
JW292_RS00375	68169..69035	+	867	WP_004118283.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2 / blaOXA-9 / blaTEM-1A / mph(E) / msr(E) / armA	-	1..108537	108537

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T194263 WP_001044770.1 NZ_CP070525:c64380-63964 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T194263 NZ_CP093400:c4659828-4659517 [Salmonella enterica subsp. enterica serovar Infantis]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT194263 WP_001261282.1 NZ_CP070525:c64607-64377 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT194263 NZ_CP093400:c4659516-4659226 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3