Detailed information of TA system TA194255

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4099020..4099723
• Replicon: chromosome
• Accession: NZ_CP070520
• Organism: Klebsiella aerogenes strain EA46506

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: JW290_RS20010
• Protein ID: WP_058647030.1
• Coordinates: 4099382..4099723 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: JW290_RS20005
• Protein ID: WP_058647031.1
• Coordinates: 4099020..4099361 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW290_RS19970	4094171..4095046	+	876	WP_058647036.1	GTPase family protein	-
JW290_RS19975	4095290..4096006	+	717	WP_058647035.1	WYL domain-containing protein	-
JW290_RS19980	4096042..4096494	+	453	WP_021571975.1	hypothetical protein	-
JW290_RS19985	4096566..4097039	+	474	WP_058647034.1	hypothetical protein	-
JW290_RS19990	4097159..4097980	+	822	WP_058647033.1	DUF945 domain-containing protein	-
JW290_RS19995	4098011..4098454	+	444	WP_058647032.1	antirestriction protein	-
JW290_RS20000	4098467..4099009	+	543	WP_080395938.1	DNA repair protein RadC	-
JW290_RS20005	4099020..4099361	+	342	WP_058647031.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JW290_RS20010	4099382..4099723	+	342	WP_058647030.1	TA system toxin CbtA family protein	Toxin
JW290_RS20015	4100298..4100858	+	561	WP_045364741.1	hypothetical protein	-
JW290_RS20020	4100855..4101196	+	342	WP_045364739.1	hypothetical protein	-
JW290_RS20025	4101237..4102106	-	870	WP_045364735.1	HNH endonuclease	-
JW290_RS20030	4102286..4102921	+	636	WP_205460336.1	HNH endonuclease	-
JW290_RS20035	4102959..4103285	-	327	WP_205460337.1	hypothetical protein	-
JW290_RS20040	4103778..4104122	-	345	WP_205460338.1	DUF1294 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4071987..4101196	29209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12707.61 Da
• Isoelectric Point: 7.1644

>T194255 WP_058647030.1 NZ_CP070520:4099382-4099723 [Klebsiella aerogenes]
MKTLPAITPQAATLCLSPVAVWQMLLTRLLEQHYGLTLNDTQFSDETVIQEHIDAGITLANAVNFLVEKYELVRIDRKGL
SWQEQSPYLRAVDILRARQATGLLRRNSSNSGL

Nucleotide

• Length: 342 bp

>T194255 NZ_CP093400:c1014583-1014056 [Salmonella enterica subsp. enterica serovar Infantis]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTACAACGCCATGCACTACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGCGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACAATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12820.44 Da
• Isoelectric Point: 5.5353

>AT194255 WP_058647031.1 NZ_CP070520:4099020-4099361 [Klebsiella aerogenes]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLYYLADRASITGEFNNAESLKLDEVLPHFISQMESMLTTGEMNPRH
AHCVTLYHNCFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 342 bp

>AT194255 NZ_CP093400:c1014870-1014580 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGACTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA