Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 32115..32758 Replicon plasmid p4605_KPC
Accession NZ_CP070519
Organism Klebsiella aerogenes strain EA46506

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag JW290_RS00185 Protein ID WP_000754566.1
Coordinates 32342..32758 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag JW290_RS00180 Protein ID WP_001261276.1
Coordinates 32115..32345 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW290_RS00145 27234..27503 + 270 WP_000339857.1 hypothetical protein -
JW290_RS00150 27680..28546 - 867 WP_004118283.1 replication initiation protein -
JW290_RS00155 29076..29180 + 105 WP_032409716.1 hypothetical protein -
JW290_RS00160 29309..29566 + 258 WP_000764642.1 hypothetical protein -
JW290_RS00165 29624..30400 - 777 WP_000015958.1 site-specific integrase -
JW290_RS00170 30397..31140 - 744 WP_000129823.1 hypothetical protein -
JW290_RS00175 31191..31541 - 351 WP_000493378.1 hypothetical protein -
JW290_RS00180 32115..32345 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JW290_RS00185 32342..32758 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
JW290_RS00190 32962..33818 + 857 Protein_37 IS3-like element ISEc15 family transposase -
JW290_RS00195 34100..35176 + 1077 WP_000227969.1 IS110 family transposase -
JW290_RS00200 35817..35900 + 84 Protein_39 SOS response-associated peptidase -
JW290_RS00205 36063..36587 - 525 Protein_40 Tn3 family transposase -
JW290_RS00210 36652..37356 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
JW290_RS00215 37411..37581 + 171 Protein_42 serine hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(6')-Ib / blaKPC-2 - 1..87618 87618


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T194243 WP_000754566.1 NZ_CP070519:32342-32758 [Klebsiella aerogenes]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T194243 NZ_CP093392:c4218335-4217844 [Salmonella enterica]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT194243 WP_001261276.1 NZ_CP070519:32115-32345 [Klebsiella aerogenes]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT194243 NZ_CP093392:c4218625-4218332 [Salmonella enterica]
ATGCCAGCCGCAAACAGTATGGCGATGAAGCGTGAAACTCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGTGCTGCCGCAGAAGAAGCGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAATTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References