Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1119951..1120573 Replicon chromosome
Accession NZ_CP070508
Organism Serratia ureilytica strain AM923

Toxin (Protein)


Gene name Hha Uniprot ID A0A8B4G7F9
Locus tag IHE77_RS05300 Protein ID WP_004940313.1
Coordinates 1119951..1120154 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2X2G5J9
Locus tag IHE77_RS05305 Protein ID WP_004940312.1
Coordinates 1120205..1120573 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHE77_RS05270 (IHE77_05280) 1115760..1116254 + 495 WP_142002001.1 type II secretion system protein GspM -
IHE77_RS05275 1116350..1116517 + 168 Protein_1011 prepilin peptidase -
IHE77_RS05280 1116548..1117054 + 507 WP_229597905.1 A24 family peptidase -
IHE77_RS05285 (IHE77_05290) 1117051..1117413 - 363 WP_052752709.1 type II secretion system pilot lipoprotein GspS -
IHE77_RS05290 (IHE77_05295) 1117671..1119098 + 1428 WP_142002003.1 N-acetylglucosamine-binding protein GbpA -
IHE77_RS05295 (IHE77_05300) 1119151..1119669 + 519 WP_033651103.1 cytochrome b/b6 domain-containing protein -
IHE77_RS05300 (IHE77_05305) 1119951..1120154 - 204 WP_004940313.1 HHA domain-containing protein Toxin
IHE77_RS05305 (IHE77_05310) 1120205..1120573 - 369 WP_004940312.1 Hha toxicity modulator TomB Antitoxin
IHE77_RS05310 (IHE77_05315) 1120733..1121086 - 354 WP_033642103.1 hypothetical protein -
IHE77_RS05315 (IHE77_05320) 1121494..1122207 + 714 WP_033642104.1 ABC transporter ATP-binding protein -
IHE77_RS05320 (IHE77_05325) 1122204..1123061 + 858 WP_033642105.1 metal ABC transporter permease -
IHE77_RS05325 (IHE77_05330) 1123087..1123965 + 879 WP_033642106.1 metal ABC transporter substrate-binding protein -
IHE77_RS05330 (IHE77_05335) 1124071..1124211 - 141 WP_004940304.1 type B 50S ribosomal protein L36 -
IHE77_RS05335 (IHE77_05340) 1124224..1124478 - 255 WP_004940303.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8107.37 Da        Isoelectric Point: 6.9756

>T194227 WP_004940313.1 NZ_CP070508:c1120154-1119951 [Serratia ureilytica]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T194227 NZ_CP093391:c4098287-4098003 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGGACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14261.95 Da        Isoelectric Point: 4.4314

>AT194227 WP_004940312.1 NZ_CP070508:c1120573-1120205 [Serratia ureilytica]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Download         Length: 369 bp

>AT194227 NZ_CP093391:c4098519-4098277 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGATA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4G7F9

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2G5J9

References