Detailed information of TA system TA194222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-DUF971
• Location: 38258..38856
• Replicon: plasmid pSDM007
• Accession: NZ_CP070507
• Organism: Pseudomonas sp. SDM007

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A2G0Y7K8
• Locus tag: JTY93_RS27685
• Protein ID: WP_042857722.1
• Coordinates: 38554..38856 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: JTY93_RS27680
• Protein ID: WP_042857721.1
• Coordinates: 38258..38557 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTY93_RS27650	34437..34868	-	432	WP_047306860.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
JTY93_RS27655	35007..35960	+	954	WP_205478554.1	non-homologous end-joining DNA ligase	-
JTY93_RS27660	35989..36264	+	276	WP_205478556.1	hypothetical protein	-
JTY93_RS27665	37362..37679	-	318	WP_042857718.1	hypothetical protein	-
JTY93_RS27670	37681..37902	-	222	WP_205480554.1	hypothetical protein	-
JTY93_RS27675	37915..38235	-	321	WP_047710214.1	hypothetical protein	-
JTY93_RS27680	38258..38557	-	300	WP_042857721.1	putative addiction module antidote protein	Antitoxin
JTY93_RS27685	38554..38856	-	303	WP_042857722.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JTY93_RS27690	39059..39838	+	780	WP_042857723.1	DUF4942 domain-containing protein	-
JTY93_RS27695	39990..40358	+	369	WP_042857724.1	hypothetical protein	-
JTY93_RS27700	40391..40708	+	318	WP_047710230.1	hypothetical protein	-
JTY93_RS27705	40768..40986	+	219	WP_182367944.1	hypothetical protein	-
JTY93_RS27710	41124..42143	+	1020	WP_205519060.1	hypothetical protein	-
JTY93_RS27715	42287..42529	+	243	WP_042857726.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib	-	1..249868	249868

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (6-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11238.81 Da
• Isoelectric Point: 10.3903

>T194222 WP_042857722.1 NZ_CP070507:c38856-38554 [Pseudomonas sp. SDM007]
MKTIKQTATYRNWERKLRDKQAKAIIAARVFRVANGLLGDVQPVGQGISELRIHHGPGYRVYFQQRGNQLVLLLCGGDKS
SQARDIETAKALASQWSDDE

Nucleotide

• Length: 303 bp

>T194222 NZ_CP093391:c942975-942448 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACAAAAACCTACGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACCGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTTTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTACTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10853.33 Da
• Isoelectric Point: 5.1084

>AT194222 WP_042857721.1 NZ_CP070507:c38557-38258 [Pseudomonas sp. SDM007]
MKEQFYDYDPADALTDAESIAVFMEDAFETGDAAYIAKCLGVVARAKGMTALANESGLSREHLYRSFSDKGNPTLKTLLA
VMRAVGLDLTTRKHVEPVH

Nucleotide

• Length: 300 bp

>AT194222 NZ_CP093391:c943262-942972 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGTGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: A0A2G0Y7K8